Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation
Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation
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DOI：
10.1007/s00425-019-03249-5
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发表时间：
2019-07
期刊：
影响因子：
4.3
通讯作者：
Tatiana V. Lapina;L. Kochemasova;K. Forchhammer;E. Ermilova
中科院分区：
文献类型：
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作者：
Tatiana V. Lapina;L. Kochemasova;K. Forchhammer;E. Ermilova
主要conclusionl-精氨酸支持增长，并导致增加PII信号蛋白水平和脂滴积累的无色绿色algaPolytomella parva.AbstractPolytomella parva，一个模型系统的非光合绿色藻类，利用铵和几个碳源，包括乙醇和乙酸。我们以前报道过P。parva积累大量的精氨酸，其中鸟氨酸/精氨酸生物合成途径的关键酶N-乙酰基-L-谷氨酸激酶表现出高活性。在这里，我们证明，l-精氨酸可以使用这种酶作为氮源。外源性精氨酸直接影响PII信号蛋白的水平和三酰甘油（TAG）填充的脂质体（LB）的形成。我们的研究结果表明，氮源，而不是氮饥饿，可能是关键的积累LB在一个PII独立的方式inP。parva。
Main conclusionl-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green algaPolytomella parva.AbstractPolytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported thatP. parvaaccumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase, exhibiting high activity. Here we demonstrate thatl-arginine can be used by this alga as a nitrogen source. Externally supplied arginine directly influenced the levels of PII signaling protein and formation of triacylglycerol (TAG)-filled lipid bodies (LBs). Our results suggest that the nitrogen source, but not nitrogen starvation, may be critical for the accumulation of LBs in a PII-independent manner inP. parva.