Acceleration of glomerulonephritis in NZB x NZW mice by early immunization with DNA and injection of bacterial lipopolysaccharide. Experimental approach to the treatment of lupus nephritis by use of the accelerated model of NZB x NZW mouse disease.
Acceleration of glomerulonephritis in NZB x NZW mice by early immunization with DNA and injection of bacterial lipopolysaccharide. Experimental approach to the treatment of lupus nephritis by use of the accelerated model of NZB x NZW mouse disease.
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通过早期 DNA 免疫和注射细菌脂多糖加速 NZB x NZW 小鼠的肾小球肾炎。
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
Contente Jj
中科院分区：
文献类型：
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作者：
Fournié Gj;Minh Mg;M. Ma;S. Hass;Lambert Ph;Contente Jj
本文研究了DNA免疫和细菌脂多糖（LPS）注射对NZB × NZW小鼠肾小球肾炎的影响。与甲基化牛血清白蛋白（DNA-mBSA）复合的DNA和LPS联合注射似乎比单独注射DNA-mBSA或LPS更有效地加速NZB x NZW小鼠的疾病。在注射DNA-mBSA和LPS的小鼠中观察到抗DNA抗体水平的快速增加、严重肾脏病变的早期出现和生存期缩短。发现这种新模型适用于用环磷酰胺和肝素治疗的患有加速疾病的小鼠的治疗研究。在小于4个月的小鼠中进行的免疫学和组织学研究显示了环磷酰胺治疗NZB x NZW小鼠疾病的疗效。肝素似乎通过防止注射DNA-mBSA和LPS诱导的毛细血管内细胞增殖而具有有益作用。NZB × NZW小鼠疾病的加速模型可能是一个有用的工具，用于实验治疗狼疮性肾炎。
The effects of early immunization with DNA and of injection of bacterial lipopolysaccharide (LPS) on the glomerulonephritis of NZB x NZW mice were studied. Combined injections of DNA complexed to methylated bovine serum albumin (DNA-mBSA) and of LPS appeared to be more efficient in accelerating the disease in NZB x NZW mice than injections of DNA-mBSA or LPS alone. A rapid increase in levels of anti-DNA antibodies, an early appearance of severe renal lesions and a shortened survival were observed in mice injected with both DNA-mBSA and LPS. This new model was found to be suitable for therapeutic studies in mice with accelerated disease treated with cyclophosphamide and heparin. The efficacy of cyclophosphamide for the treatment of NZB x NZW mouse disease was shown by immunological and histological studies in mice younger than 4 months. Heparin appeared to have a beneficial effect by preventing the endocapillary cellular proliferation induced by injections of DNA-mBSA and LPS. The accelerated model of NZB x NZW mouse disease might be a useful tool for experiments on the treatment of lupus nephritis.