hucMSC derived exosomes promote functional recovery in spinal cord injury mice via attenuating inflammation
hucMSC derived exosomes promote functional recovery in spinal cord injury mice via attenuating inflammation
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HucMSC衍生的外泌体通过减轻炎症促进脊髓损伤小鼠的功能恢复
DOI：
10.1016/j.msec.2018.04.006
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发表时间：
2018-08-01
影响因子：
7.9
通讯作者：
Wang, Baocheng
中科院分区：
文献类型：
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作者：
Sun, Guodong;Li, Guangqiang;Wang, Baocheng
由于脊髓损伤（spinal cord injury，SCI）发病率高、病理复杂、靶点不明确，寻求有效的治疗方法仍是一个巨大的挑战。人脐带间充质干细胞（human umbilical cord mesenchymal stem cells，hucMSC）在组织再生中发挥重要作用。然而，移植干细胞有潜在的致畸风险。最近的研究表明，干细胞分泌的外泌体可能有助于组织损伤修复。我们假设hucMSC衍生的外泌体的应用可能是SCI治疗的潜在方式。我们的研究显示，平均粒径为70 nm的hucMSC衍生的外泌体可以有效地触发骨髓衍生的巨噬细胞（BMDM）从M1极化为M2表型。体内研究表明，hucMSC衍生的外泌体可以通过下调炎性细胞因子如TNF-α、MIP-1 α、IL-6和IFN-γ来改善SCI后的功能恢复。总的来说，我们的研究结果表明，hucMSC衍生的外泌体可以通过减轻损伤区域的炎症来促进脊髓损伤愈合。我们的研究结果为hucMSC衍生的外泌体在软组织修复中的应用提供了新的视角和治疗策略。
The exploration of effective spinal cord injury (SCI) healing still remain a great challenge due to the high morbidity, complex pathology and unclear targets. Human umbilical cord mesenchymal stem cells (hucMSC) play an important role in tissue regeneration. However, transplanting stem cells has a potential risk of teratogenicity. Recent studies have suggested that exosomes secreted by stem cells may contribute to tissue injury repair. We hypothesized that the application of hucMSC derived exosomes may be a potential way for SCI treatment. Our studies showed the hucMSC derived exosomes with a mean particle size of 70 nm could effectively trigger the bone marrow derived macrophage (BMDM) polarization from M1 to a M2 phenotype. In vivo studies demonstrated that the hucMSC derived exosomes could improve the functional recovery after SCI through down-regulation of the inflammatory cytokines, such as TNF-alpha, MIP-1 alpha, IL-6 and IFN-gamma. Collectively, our findings indicated that hucMSC derived exosomes could facilitate spinal cord injury healing via attenuating the inflammation of the injury region. Our results provided a new perspective and therapeutic strategy for the use of hucMSC derived exosomes in soft tissue repair.