AN IMMUNOFLUORESCENT SURVEY OF THE BROWN TIDE CHRYSOPHYTE AUREOCOCCUS-ANOPHAGEFFERENS ALONG THE NORTHEAST COAST OF THE UNITED-STATES
AN IMMUNOFLUORESCENT SURVEY OF THE BROWN TIDE CHRYSOPHYTE AUREOCOCCUS-ANOPHAGEFFERENS ALONG THE NORTHEAST COAST OF THE UNITED-STATES
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DOI：
10.1093/plankt/15.5.563
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发表时间：
1993-05-01
影响因子：
2.1
通讯作者：
NUZZI, R
中科院分区：
文献类型：
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作者：
ANDERSON, DM;KEAFER, BA;NUZZI, R
1988年和1990年，在美国东北海岸，在NH的朴茨茅斯和切萨皮克湾之间进行了调查，以确定金黄色葡萄球菌的种群分布。金黄色葡萄球菌是从1985年开始在长岛和纳拉甘西特湾造成大规模和破坏性‘褐潮’的金藻。一种特定物种的免疫荧光技术被用来筛选水样，在低至10-20个细胞毫升-1的细胞浓度下，阳性鉴定是可能的。这两年，在新泽西州长岛和巴尼加特湾及其周围的许多站点都检测到厌食弧菌，通常细胞浓度很高。在这个“中心”的北部和南部，其余近一半的站对厌食杆菌呈阳性，但细胞总是处于非常低的细胞浓度。在远离长岛的地区，许多阳性鉴定是在没有已知有害赤潮历史的水域进行的。该物种存在于开阔的沿海和河口位置，盐度在18至32个实际盐度单位(PSU)之间。观察到的种群分布显然仍然反映了1985年该物种首次爆发时的大规模疫情，因为在长岛附近有大量阳性地点和高丰度的厌食性嗜热菌。然而，这种物种在远离种群中心的水域频繁出现，令人不安。厌食金黄色葡萄球菌的分布比之前认为的更广泛。因此，许多地区有可能出现破坏性的褐潮，例如与1985年该物种突然出现有关的那些。
Surveys were conducted along the northeast coast of the USA, between Portsmouth, NH, and the Chesapeake Bay in 1988 and 1990, to determine the population distribution of Aureococcus anophagefferens, the chrysophyte responsible for massive and destructive 'brown tides' in Long Island and Narragansett Bay beginning in 1985. A species-specific immunofluorescent technique was used to screen water samples, with positive identification possible at cell concentrations as low as 10-20 cells ml-1. Both years, A.anophagefferens was detected at numerous stations in and around Long Island and Barnegat Bay, NJ, typically at high cell concentrations. To the north and south of this 'center', nearly half of the remaining stations were positive for A.anophagefferens, but the cells were always at very low cell concentrations. Many of the positive identifications in areas distant from Long Island were in waters with no known history of harmful brown tides. The species was present in both open coastal and estuarine locations, in salinities between 18 and 32 practical salinity units (PSU). The observed population distributions apparently still reflect the massive 1985 outbreak when this species first bloomed, given the number of positive locations and high abundance of A.anophagefferens in the immediate vicinity of Long Island. However, the frequent occurrence of this species in waters far from this population 'center' is disturbing. Aureococcus anophagefferens is more widely distributed than was previously thought. Numerous areas thus have the potential for destructive brown tides such as those associated with the sudden appearance of the species in 1985.