PHOSPHATIDYLGLYCEROL IS INVOLVED IN PROTEIN TRANSLOCATION ACROSS ESCHERICHIA-COLI INNER MEMBRANES
PHOSPHATIDYLGLYCEROL IS INVOLVED IN PROTEIN TRANSLOCATION ACROSS ESCHERICHIA-COLI INNER MEMBRANES
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DOI：
10.1038/334173a0
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发表时间：
1988-07-14
期刊：
影响因子：
64.8
通讯作者：
DEKRUIJFF, B
中科院分区：
文献类型：
--
作者：
DEVRIJE, T;DESWART, RL;DEKRUIJFF, B
新合成的蛋白质要从细菌细胞的细胞质中输出，必须穿过内膜。在革兰氏阴性细菌 ATP1,2 中，需要膜电位 3,4、secgenes5 和前导肽酶 6,7（切割前体蛋白 N 末端信号肽的酶）的产物。然而，易位机制仍然难以捉摸。膜脂的重要附加作用已在理论基础8-11和模型系统实验的基础上被反复提出12-14，但尚未获得直接证据。我们在这里证明，使用大肠杆菌突变体来合成主要阴离子膜磷脂，磷脂酰甘油参与新合成的外膜蛋白穿过内膜的易位。
Newly synthesized proteins to be exported out of the cytoplasm of bacterial cells have to pass across the inner membrane. In Gram-negative bacteria ATP1,2, a membrane potential3,4, the products of thesecgenes5and leader peptidases6,7(enzymes which cleave the N-terminal signal peptides of the precursor proteins) are required. The mechanism of translocation, however, remains elusive. Important additional roles for membrane lipids have been repeatedly suggested both on theoretical grounds8–11and on the basis of experiments with model systems12–14but no direct evidence had been obtained. We demonstrate here, using mutants ofEscherichia colidefective in the synthesis of the major anionic membrane phospholipids, that phosphatidylglycerol is involved in the translocation of newly synthesized outer-membrane proteins across the inner membrane.