FORMATION OF INFECTIOUS PROGENY VIRUS AFTER INSERTION OF HERPES-SIMPLEX THYMIDINE KINASE GENE INTO DNA OF AN AVIAN RETROVIRUS
FORMATION OF INFECTIOUS PROGENY VIRUS AFTER INSERTION OF HERPES-SIMPLEX THYMIDINE KINASE GENE INTO DNA OF AN AVIAN RETROVIRUS
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DOI：
10.1016/0092-8674(81)90034-9
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
TEMIN, HM
中科院分区：
文献类型：
--
作者：
SHIMOTOHNO, K;TEMIN, HM
制备了几种含有单纯疱疹病毒1型胸苷激酶（tk）基因的禽逆转录病毒、脾坏死病毒的传染性原液。将克隆的脾坏死病毒与 tk DNA 和克隆的网状内皮增生病毒 A 株的 DNA 重组体的 DNA 共转染鸡细胞后产生病毒。去除 tk 基因中 tk mRNA 末端的序列，感染性重组病毒的产量增加了 1000 倍。用重组病毒感染鸡或大鼠的 tk- 细胞，将其转化为 tk+ 表型。
Several infectious stocks of an avian retrovirus, spleen necrosis virus, containing the herpes simplex virus type 1 thymidine kinase (tk) gene were prepared. The viruses were produced after cotransfection of chicken cells with DNA from recombinants between cloned spleen necrosis virus and tk DNA and DNA of cloned reticuloendotheliosis virus strain A. Removal of sequences in the tk gene for the end of tk mRNA increased 1000-fold the yield of infectious recombinant virus. Infection of chicken or rat tk- cells with the recombinant virus transformed them to a tk+ phenotype.