Hollow double-layered polymer nanoparticles with S-nitrosothiols for tumor targeted therapy
Hollow double-layered polymer nanoparticles with S-nitrosothiols for tumor targeted therapy
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含S-亚硝基硫醇的空心双层聚合物纳米粒子用于肿瘤靶向治疗
DOI：
10.1039/c7tb01715d
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发表时间：
2017-09-28
影响因子：
7
通讯作者：
Yang, Xinlin
中科院分区：
文献类型：
--
作者：
Liu, Tuanwei;Hu, Jingjing;Yang, Xinlin
描述了具有用于释放一氧化氮（NO）的S-亚硝基硫醇的肿瘤靶向中空双层聚合物纳米颗粒（HDPN）作为化疗。通过两段蒸馏沉淀共聚合、简单的后处理和S-亚硝基硫醇修饰，S-亚硝基HDPN表现出pH和葡萄糖的双重响应性。这将有利于与唾液酸过表达的癌细胞准确结合，为二硫键聚合物辅助细胞摄取、细胞内GSH诱导的分解和快速NO释放提供先决条件。共聚焦显微镜和细胞毒性试验表明，正常与肿瘤细胞的体外识别，细胞内的交付能力和肿瘤细胞靶向细胞毒性。特别值得一提的是，NO在传输过程中不可避免的少量泄漏，参与正常的生理活动，不会引起严重的副作用，为避免传统化疗治疗癌症的不可耐受的副作用提供了一种可能的解决方案。
Tumor targeted hollow double-layered polymer nanoparticles (HDPNs) with S-nitrosothiols for nitric oxide (NO)-release as chemotherapy were described. Via a two-stage distillation precipitation co-polymerization, simple post-treatment and S-nitrosothiol modification, the S-nitroso HDPNs showed pH and glucose dual responsiveness. This would benefit accurate binding with the sialic acid over-expressed cancer cells, providing prerequisites for the disulfide polymer assisted cell uptake, intracellular GSH induced decomposition and rapid NO release. Confocal microscopy and cytotoxicity assay with normal versus tumor cells demonstrated in vitro recognition, intracellular delivery ability and tumor cell targeting cytotoxicity. Especially worth mentioning, the inevitable small amount of NO leakage in the transmission would take part in normal physiological activities and not cause serious side effects, providing a possible solution to avoid the intolerable side effects of traditional chemotherapy treatments for cancer.