Inorganic Carbon Assimilation and Electrosynthesis of Platform Chemicals in Bioelectrochemical Systems (BESs) Inoculated with Clostridium saccharoperbutylacetonicum N1-H4.
Inorganic Carbon Assimilation and Electrosynthesis of Platform Chemicals in Bioelectrochemical Systems (BESs) Inoculated with Clostridium saccharoperbutylacetonicum N1-H4.
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DOI：
10.3390/microorganisms11030735
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发表时间：
2023-03-13
期刊：
影响因子：
4.5
通讯作者：
Rossa CA
中科院分区：
文献类型：
--
作者：
Nastro RA;Salvian A;Kuppam C;Pasquale V;Pietrelli A;Rossa CA
为了满足平台化学品的需求，需要更绿色的工艺，以及重新利用人类活动产生的CO2的可能性，最近鼓励了对生物电化学系统（BES）的建立，优化和开发的研究，用于从无机碳（CO2，HCO 3 −）电合成有机化合物。在本研究中，我们测试了糖多丁基丙酮梭菌N1-4（DSMZ 14923）从CO2：N2气体混合物中存在的无机碳产生乙酸盐和D-3-羟基丁酸盐的能力。同时，我们测试了希瓦氏菌（Shewanella oneidensis）MR 1和铜绿假单胞菌（Pseudomonas aeruginosa）PA 1430/CO 1聚生体提供还原能力以维持阴极碳同化的能力。我们测试了具有相同布局、接种物和培养基的三种不同系统的性能，但施加了1.5 V外部电压、1000 mA外部负载，并且电极或外部设备之间没有任何连接（开路电压，OCV）。我们比较了CO2同化率和生产的代谢产物（甲酸，乙酸3-D-羟基丁酸）在我们的BES与非产电控制文化中获得的值，并估计所使用的能量由我们的BES同化1摩尔的CO2。结果表明，C.当微生物燃料电池（MFC）连接到1000 μ m的外部电阻器时，以希瓦氏菌/假单胞菌聚生体作为唯一的电子源，糖多丁基丙酮菌NT-1实现了最大的CO2同化（95.5%）。此外，我们检测到C代谢的转变。糖多丁基丙酮NT-1，因为其在BES中的延长活性。我们的研究结果为BES在碳捕获和平台化学品电合成中的应用开辟了新的前景。
The need for greener processes to satisfy the demand of platform chemicals together with the possibility of reusing CO2 from human activities has recently encouraged research on the set-up, optimization, and development of bioelectrochemical systems (BESs) for the electrosynthesis of organic compounds from inorganic carbon (CO2, HCO3−). In the present study, we tested the ability of Clostridium saccharoperbutylacetonicum N1-4 (DSMZ 14923) to produce acetate and D-3-hydroxybutyrate from inorganic carbon present in a CO2:N2 gas mix. At the same time, we tested the ability of a Shewanella oneidensis MR1 and Pseudomonas aeruginosa PA1430/CO1 consortium to provide reducing power to sustain carbon assimilation at the cathode. We tested the performance of three different systems with the same layouts, inocula, and media, but with the application of 1.5 V external voltage, of a 1000 Ω external load, and without any connection between the electrodes or external devices (open circuit voltage, OCV). We compared both CO2 assimilation rate and production of metabolites (formate, acetate 3-D-hydroxybutyrate) in our BESs with the values obtained in non-electrogenic control cultures and estimated the energy used by our BESs to assimilate 1 mol of CO2. Our results showed that C. saccharoperbutylacetonicum NT-1 achieved the maximum CO2 assimilation (95.5%) when the microbial fuel cells (MFCs) were connected to the 1000 Ω external resistor, with the Shewanella/Pseudomonas consortium as the only source of electrons. Furthermore, we detected a shift in the metabolism of C. saccharoperbutylacetonicum NT-1 because of its prolonged activity in BESs. Our results open new perspectives for the utilization of BESs in carbon capture and electrosynthesis of platform chemicals.
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