Characterization of the gut microbiota of Kawasaki disease patients by metagenomic analysis.
Characterization of the gut microbiota of Kawasaki disease patients by metagenomic analysis.
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DOI：
10.3389/fmicb.2015.00824
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发表时间：
2015
影响因子：
5.2
通讯作者：
Kuroda M
中科院分区：
文献类型：
--
作者：
Kinumaki A;Sekizuka T;Hamada H;Kato K;Yamashita A;Kuroda M
川崎病是一种发生于儿童早期的急性发热性疾病。以前的报告表明，遗传性疾病的易感因素，以及触发感染剂，可能参与KD的发病机制，然而，这种疾病的确切病因仍然未知。此外，先前基于培养的研究表明肠道微生物群在KD发病机制中可能起作用。在这项研究中，我们进行了宏基因组分析，以全面评估28名KD患者肠道菌群的纵向变化。几个显着的细菌属通常提取在急性期，而在KD的非急性期观察到瘤胃球菌属细菌的数量相对增加。基于菌种分类的宏基因组分析结果表明，与5种链球菌具有相似性的测序读段数为100个。（S.肺炎、假性肺炎、口腔链球菌、戈登氏链球菌和血链球菌），以及患者来源的链球菌分离株，在大多数患者的急性期显著增加。链球菌包括多种促进人类健康的致病菌和益生菌;因此，这种进一步的物种区分可以全面阐明KD相关的微生物群。本研究结果提示，KD相关链球菌可能参与了该病的发病机制。
Kawasaki disease (KD) is an acute febrile illness of early childhood. Previous reports have suggested that genetic disease susceptibility factors, together with a triggering infectious agent, could be involved in KD pathogenesis; however, the precise etiology of this disease remains unknown. Additionally, previous culture-based studies have suggested a possible role of intestinal microbiota in KD pathogenesis. In this study, we performed metagenomic analysis to comprehensively assess the longitudinal variation in the intestinal microbiota of 28 KD patients. Several notable bacterial genera were commonly extracted during the acute phase, whereas a relative increase in the number of Ruminococcus bacteria was observed during the non-acute phase of KD. The metagenomic analysis results based on bacterial species classification suggested that the number of sequencing reads with similarity to five Streptococcus spp. (S. pneumonia, pseudopneumoniae, oralis, gordonii, and sanguinis), in addition to patient-derived Streptococcus isolates, markedly increased during the acute phase in most patients. Streptococci include a variety of pathogenic bacteria and probiotic bacteria that promote human health; therefore, this further species discrimination could comprehensively illuminate the KD-associated microbiota. The findings of this study suggest that KD-related Streptococci might be involved in the pathogenesis of this disease.