Expression of Ras homologous B protein in the human scalp skin and hair follicles: hair follicle cycle stages-associated changes
Expression of Ras homologous B protein in the human scalp skin and hair follicles: hair follicle cycle stages-associated changes
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DOI：
10.1111/j.1600-0560.2009.01457.x
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发表时间：
2010-07-01
影响因子：
1.7
通讯作者：
Hussein, Mahmoud Rezk A.
中科院分区：
文献类型：
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作者：
Adly, Mohamed A.;Assaf, Hanan A.;Hussein, Mahmoud Rezk A.
目的：验证RhoB蛋白在人皮肤中表达，并且其表达经历毛囊周期依赖性变化的假说。为了验证这一假设，我们用免疫组织化学方法检测了RhoB在正常人皮肤和毛囊(HFs)中的表达。方法：50例正常人头皮皮肤标本来自50名女性(年龄53-57岁)，接受择期整形手术。标本取自头皮的额部和颞部。用免疫组织化学方法检测50例HF，其中生长期35例，退化期10例，休止期5例。结果：RhoB蛋白在人头皮、皮肤和毛囊的各种成分中均有较强的表达。在表皮中，除角质层完全无RhoB蛋白外，其余各层均呈中等程度的RhoB免疫反应。在皮脂腺中，所有皮脂腺细胞均呈强的RhoB免疫反应。在毛囊中，RhoB蛋白的表达呈现出与毛囊周期阶段相关的变化，即生长期表达较强，成熟期和成熟期表达较弱或完全缺失。半定量分析显示，生长期VI毛囊的表达强度、阳性细胞百分率和免疫反应评分均显著高于生长期毛囊和生长期毛囊(p<；0.05)。同样，RhoB蛋白在基底层、棘层和皮脂腺中的表达显著高于颗粒层(p<；0.05)。结论：我们首次报道了RhoB蛋白在人头皮皮肤和毛囊中的分布。我们还首次指出，在毛发周期的不同阶段，该蛋白的表达存在差异。Adly M.A，Assaf H.A，Hussein M.R.A.：RAS同源B蛋白在人头皮皮肤和毛囊中的表达：毛囊周期阶段相关的变化。
Aim: To test the hypothesis that 'RhoB protein is expressed in the human skin and its expression undergoes hair follicle cycle dependent changes'. To test this hypothesis, we examined the expression of RhoB in the normal human skin and hair follicles (HFs) using immunohistochemical methods.Methods: A total of 50 normal human scalp skin specimens were obtained from 50 females (age: 53-57 years) undergoing elective cosmetic plastic surgery. The specimens were obtained from both frontal and temporal regions of the scalp. A total of 50 HF, (35 anagen, 10 catagen and 5 telogen) were examined in each case using immunohistologic staining methods. Semiquantitative analysis was done.Results: RhoB protein was strongly expressed in the various elements of the human scalp skin and hair follicles. In the epidermis, a moderate RhoB immunoreactivity was found in all layers except stratum corneum where RhoB protein was completely absent. In sebaceous glands, a strong RhoB immunoreactivity was detected in all sebaceocytes. In the hair follicles, the expression of RhoB protein showed hair follicle cycle stages-associated changes, i.e. strong expression during anagen, but weak and completely absent expressions during catagen and telogen phases, respectively. Semiquantitative analysis revealed statistically significant high expression values (staining intensity, percentage of positive cells and immunoreactivity scores) in the anagen VI hair follicles compared to either cantagen or telogen ones (p < 0.05). Similarly, RhoB protein expression was significantly high in the stratum basale, stratum spinosum and sebaceous glands compared to stratum granulosum (p < 0.05).Conclusions: Here we report, for the first time, the distribution of RhoB protein in the human scalp skin and hair follicles. We also provide the first indication that there are variations in the expression of this protein in the different stages of the hair cycle.Adly M.A, Assaf H.A, Hussein M.R.A. Expression of Ras homologous B protein in the human scalp skin and hair follicles: hair follicle cycle stages-associated changes.