A survey of indigenous microbial hydrocarbon degradation genes in soils from Antarctica and Brazil
A survey of indigenous microbial hydrocarbon degradation genes in soils from Antarctica and Brazil
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DOI：
10.1139/w04-008
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发表时间：
2004-05-01
影响因子：
2.8
通讯作者：
Greer, CW
中科院分区：
文献类型：
--
作者：
Luz, AP;Pellizari, VH;Greer, CW
对来自南极洲和巴西的 29 个未污染土壤以及受石油烃和氯有机物影响的土壤的总群落 DNA 进行了筛选，以确定是否存在来自已知细菌生物降解途径的九种分解代谢基因，这些基因编码烷烃单加氧酶或芳香族双加氧酶。针对烷烃单加氧酶基因的特异性引物和探针源自恶臭假单胞菌 ATCC 29347 (Pp alkB)、红球菌属。菌株 Q15（Rh alkB1、Rh alkB2）和不动杆菌属。 ADP-1（Ac alkM）。此外，检测芳香族双加氧酶基因的引物和探针源自恶臭假单胞菌 ATCC 17484 (ndoB)、恶臭假单胞菌 F1 (todC1)、恶臭假单胞菌 ATCC 33015 (xylE 和 cat23) 和假产碱假单胞菌 KF707 (bphA)。引物和探针用于通过 PCR 和杂交分析来分析总群落 DNA 提取物。除 Ac alkM 外，所有分解代谢基因均在两个地理区域的污染土壤和对照土壤中检测到，其中在南极土壤中出现频率较高。烷烃单加氧酶基因 Rh alkB1 和 Rh alkB2 是这两个地区最常检测到的 alk 基因，而 Pp alkB 在巴西土壤中未检测到。编码芳香族双加氧酶甲苯双加氧酶 (todC1) 和联苯二加氧酶 (bphA) 的基因在南极洲最常见，而 todC1 和儿茶酚-2,3-双加氧酶 (cat23) 在巴西土壤中最常见。杂交分析证实了PCR结果，表明所使用的探针与土壤提取物中检测到的基因具有高度同源性，并且能够有效检测本土微生物群体的生物降解潜力。
Total community DNA from 29 noncontaminated soils and soils impacted by petroleum hydrocarbons and chloro-organics from Antarctica and Brazil were screened for the presence of nine catabolic genes, encoding alkane monooxygenase or aromatic dioxygenases, from known bacterial biodegradation pathways. Specific primers and probes targeting alkane monooxygenase genes were derived from Pseudomonas putida ATCC 29347 (Pp alkB), Rhodococcus sp. strain Q15 (Rh alkB1, Rh alkB2), and Acinetobacter sp. ADP-1 (Ac alkM). In addition, primers and probes detecting aromatic dioxygenase genes were derived from P. putida ATCC 17484 (ndoB), P. putida F1 (todC1), P. putida ATCC 33015 (xylE and cat23), and P. pseudoalcaligenes KF707 (bphA). The primers and probes were used to analyze total community DNA extracts by using PCR and hybridization analysis. All the catabolic genes, except the Ac alkM, were detected in contaminated and control soils from both geographic regions, with a higher frequency in the Antarctic soils. The alkane monooxygenase genes, Rh alkB1 and Rh alkB2, were the most frequently detected alk genes in both regions, while Pp alkB was not detected in Brazil soils. Genes encoding the aromatic dioxygenases toluene dioxygenase (todC1) and biphenyl dioxygenase (bphA) were the most frequently detected in Antarctica, and todC1 and catechol-2,3-dioxygenase (cat23) were the most frequent in Brazil soils. Hybridization analysis confirmed the PCR results, indicating that the probes used had a high degree of homology to the genes detected in the soil extracts and were effective in detecting biodegradative potential in the indigenous microbial population.