Surface-Enhanced Raman Scattering Bioimaging with an Ultrahigh Signal-to-Background Ratio under Ambient Light
Surface-Enhanced Raman Scattering Bioimaging with an Ultrahigh Signal-to-Background Ratio under Ambient Light
复制标题
环境光下具有超高信背景比的表面增强拉曼散射生物成像
DOI：
10.1021/acsami.2c01063
复制
发表时间：
2022
影响因子：
9.5
通讯作者：
Jian Ye
中科院分区：
文献类型：
--
作者：
Shuo Zhu;Binge Deng;Fangbing Liu;Jing Li;Li Lin;Jian Ye
表面增强拉曼散射（Sers）纳米探针由于其高灵敏度和指纹谱的特异性而在生物成像领域引起了特别的兴趣。然而，Sers成像中有限的信号背景比（SBR）和在黑暗环境中执行成像的要求在很大程度上阻碍了其生物医学应用。为了解决这个问题，我们开发了一种用于Sers成像的生物正交纳米探针，具有良好的SBR和环境光抗干扰能力。核-壳纳米探针表现出强烈增强的拉曼信号，并抑制来自金属纳米颗粒的非共振激发的光致发光以及来自组织的近红外激光激发的拉曼散射和自发荧光的背景。这种纳米探针在Sers生物成像中实现了超过100的SBR，比传统的共振纳米探针高5倍，并且它们在拉曼沉默区的生物正交信号在环境光下呈现抗干扰能力。这些Sers探针的发展为拉曼成像在临床医学中的未来应用开辟了一个新的时代。
Surface-enhanced Raman scattering (SERS) nanoprobes have attracted particular interests in the field of bioimaging owing to their high sensitivity and specificity of the fingerprint spectrum. However, the limited signal-to-background ratio (SBR) in SERS imaging and the requirement to perform imaging in a dark environment have largely hindered its biomedical application. To circumvent this, we have developed a type of bio-orthogonal nanoprobes for SERS imaging with an ultrahigh SBR and ambient light anti-interference ability. The core–shell nanoprobes exhibit strongly enhanced Raman signals and depress the background from photoluminescence of metallic nanoparticles by off-resonance excitation and from the Raman scattering and auto-fluorescence of tissues by near-infrared laser excitation. Such nanoprobes have achieved an SBR of over 100 in SERS bioimaging, 5 times higher than the traditional on-resonant nanoprobes, and their bio-orthogonal signal in the Raman-silent region renders the anti-interference capability under ambient light. The development of these SERS probes opens up a new era for the future applications of Raman imaging in clinical medicine.