Identification of the equine herpesvirus type 1 glycoprotein 17/18 as a homologue of herpes simplex virus glycoprotein D.
Identification of the equine herpesvirus type 1 glycoprotein 17/18 as a homologue of herpes simplex virus glycoprotein D.
复制标题
DOI：
10.1099/0022-1317-73-5-1227
复制
发表时间：
1992-05
期刊：
影响因子：
--
通讯作者：
D. Elton;I. Halliburton;R. A. Killington;D. Meredith;W. Bonass
中科院分区：
文献类型：
--
作者：
D. Elton;I. Halliburton;R. A. Killington;D. Meredith;W. Bonass
已经确定了 Ab1 株马疱疹病毒 1 型 (EHV-1) gD 基因同源物的 DNA 序列，并与之前公布的序列进行了比较。该基因的一部分已定位于基因组的一个区域，该区域也编码单纯疱疹病毒1型基因gE和gI的同源物，并且已知编码病毒体蛋白gp17/18的表位。通过 DNA 杂交对 EHV-1 菌株 Kentucky A (KyA) 进行的分析表明存在 gD 基因同源物，并确定不存在 gI 和 gE 基因。然而，蛋白质印迹分析显示KyA病毒颗粒含有gp17/18，从而表明该蛋白是由gD基因同源物编码的。发现 KyA gp17/18 比其他菌株中检测到的小，这是由于 KyA 序列相对于 Ab1 的移码突变所致。 KyA 菌株中的突变导致 C 末端序列发生改变，并且可以解释与单克隆抗体 (MAb) 的反应所暗示的明显结构差异。我们还在大肠杆菌中将 Ab1 gD 基因的一部分表达为与谷胱甘肽 S-转移酶的融合蛋白，并表明它与最初用于绘制 gp17/18 图谱的 MAb 5H6 发生反应。这些实验证实 gp17/18 由 gD 基因同源物编码。
The DNA sequence of the equine herpesvirus type 1 (EHV-1) gD gene homologue has been determined for the strain Ab1 and compared with previously published sequences. A portion of the gene has been located to a region of the genome which also encodes homologues of the herpes simplex virus type 1 genes for gE and gI and is known to encode an epitope of the virion protein gp17/18. Analysis of the EHV-1 strain Kentucky A (KyA) by DNA hybridization showed the presence of a gD gene homologue and established the absence of genes for gI and gE. Western blot analysis, however, showed that KyA virus particles contain gp17/18, thus indicating that this protein is encoded by the gD gene homologue. The KyA gp17/18 was found to be smaller than that detected in other strains and this is accounted for by a frameshift mutation in the KyA sequence relative to Ab1. The mutation in the KyA strain results in an altered C-terminal sequence and could explain the apparent structural differences suggested by the reactivities with monoclonal antibodies (MAbs). We have also expressed part of the Ab1 gD gene as a fusion protein with glutathione S-transferase in Escherichia coli and shown that this reacts with the MAb 5H6 originally used to map gp17/18. These experiments establish that gp17/18 is encoded by the gD gene homologue.