Bacterial activities in shallow lakes – a comparison between extremely acidic and alkaline eutrophic hard water lakes
Bacterial activities in shallow lakes – a comparison between extremely acidic and alkaline eutrophic hard water lakes
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浅水湖泊中的细菌活动——极酸性和碱性富营养化硬水湖泊的比较
DOI：
10.1023/b:hydr.0000008623.73250.c8
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发表时间：
2003
期刊：
影响因子：
2.6
通讯作者：
J. Jander
中科院分区：
文献类型：
--
作者：
B. Nixdorf;J. Jander
通过 DAPI 染色和 3H-胸苷掺入方法对来自德国东北部非常浅的肥厚硬水湖和酸性采矿湖的样品中的细菌丰度和活性进行了研究。酸性湖泊中的细菌细胞数量比富营养化的天然湖泊中的细菌细胞数量大约少一个数量级，但由于方法学问题可能被低估。浅酸性采矿湖的细菌活性很高，与富营养化硬水湖的细菌活性相当。通过应用显微放射自显影技术，我们检查了酸性水中胸苷的高吸收率是否是由于复杂的化学过程造成的人为因素造成的。定性分析表明，除了小球菌和杆状细菌外，浅酸性湖中的大型丝状细菌也有助于胸苷的高吸收。我们观察到深层和二聚酸性采矿湖中的细菌活性明显较低。这种现象不能仅用营养状态来解释。据推测，浅度与地下水输入、强烈的沉积物-水相互作用、不良的浮游动物定植和光解过程相结合，有利于浅酸性采矿湖中具有高比活性的大型细菌细胞的发育。
Bacterial abundance and activity were investigated by DAPI-staining and 3H-thymidine incorporation method in samples from a very shallow, hypertrophic hard water lake and from acidic mining lakes in north-eastern Germany. Bacterial cell numbers in the acidic lakes were about one order of magnitude less than in the hypertrophic natural lake but are probably underestimated due to methodological problems. Bacterial activity in the shallow acidic mining lakes was high and comparable with that of hypertrophic hard water lakes. By application of microautoradiography, we checked whether these high thymidine uptake rates in acidic waters were caused by artefacts due to the complex chemistry. It was shown by this qualitative analysis that in addition to small cocci and rods, large filamentous bacteria in the shallow acidic lakes also contribute to the high thymidine uptake. We observed clearly lower bacterial activities in deep and dimictic acidic mining lakes. This phenomenon cannot be explained by the trophic state alone. It is assumed that shallowness in combination with the groundwater input, intense sediment–water interaction, the poor zooplankton colonization and photolytic processes favours the development of large bacterial cells with high specific activity in shallow acidic mining lakes.