EXPRESSION OF THE LEISHMANIA-TARENTOLAE UBIQUITIN-ENCODING AND MINI-EXON GENES
EXPRESSION OF THE LEISHMANIA-TARENTOLAE UBIQUITIN-ENCODING AND MINI-EXON GENES
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DOI：
10.1016/0378-1119(94)90201-1
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发表时间：
1994-06-24
期刊：
影响因子：
3.5
通讯作者：
CAMPBELL, DA
中科院分区：
文献类型：
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作者：
FLEISCHMANN, J;CAMPBELL, DA
为了开发动质体原生动物中的转录和反式剪接模型，我们对塔伦托利什曼原虫 (Lt) 中的泛素 (Ubi) 基因组织和 mRNA 加工进行了表征。鉴定了三个 ubi 位点：两个离散的 Ubi 延伸蛋白 52 (EP52) 编码基因（ubiA 和 ubiB）和一个多态性多泛素编码基因 (ubiC)。这三个位点分别位于2.05 Mb、630 kb 和2.9 Mb 的染色体上。根据上游侧翼基因同一性，ubiB 似乎是布氏锥虫 (Tb) 中串联重复的 ubi-EP52/1 和 2 的同源物。与克氏锥虫类似，Lt 不包含在酿酒酵母和多细胞生物中发现的 ubi-EP76 同源物。所有三个 Lt ubi 位点均被转录。来自 ubi 位点的初级转录本在 5' 端通过与迷你外显子的反式剪接进行处理。还对产生 95 nt 初级转录本（转拼反应中的第二个模板）的 Lt 迷你外显子基因 (min) 进行了表征。
To develop models for transcription and trans-splicing in kinetoplastid protozoa, we have characterized ubiquitin (Ubi) gene organization and mRNA processing in Leishmania tarentolae (Lt). Three ubi loci were characterized: two discrete Ubi-extension protein 52 (EP52)-encoding genes (ubiA and ubiB) and a polymorphic polyubiquitin-encoding gene (ubiC). The three loci resided on chromosomes of 2.05 Mb, 630 kb and 2.9 Mb, respectively. On the basis of upstream flanking gene identity, ubiB appears to be the homologue of the tandemly repeated ubi-EP52/1 and 2 in Trypanosoma brucei (Tb). Similar to Trypanosoma cruzi, Lt did not contain a homologue of the ubi-EP76 that has been found in Saccharomyces cerevisiae and multicellular organisms. All three Lt ubi loci were transcribed. The primary transcripts from the ubi loci were processed at the 5'-end by trans-splicing with the mini-exon. A Lt mini-exon gene (min) that gave rise to a 95-nt primary transcript, which is the second template in the trans-splicing reaction, was also characterized.