MPF2-Like MADS-Box Genes Affecting Expression of SOC1 and MAF1 are Recruited to Control Flowering Time
MPF2-Like MADS-Box Genes Affecting Expression of SOC1 and MAF1 are Recruited to Control Flowering Time
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DOI：
10.1007/s12033-012-9540-9
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发表时间：
2013-05-01
影响因子：
2.6
通讯作者：
Ali, Ghulam Muhammad
中科院分区：
文献类型：
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作者：
Khan, Muhammad Ramzan;Khan, Irfan Ullah;Ali, Ghulam Muhammad
植物的成花转变是一个复杂的基因网络，它响应各种发育和环境信号。MADS-box基因是决定植物开花时间的关键调控因子和主要贡献者。以前，属于STMADS 11超进化枝的MPF 2样基因在Withania（WSA 206和WSB 206）和Tubocapsicum（TAB 201）中被复制成MPF 2样A和MPF 2样B。本研究通过分析35 S：MPF 2-like转基因拟南芥植株来确定MPF 2-like基因对开花时间的影响，并探讨它们对SOC 1（一种成花启动子）和MAF 1（一种成花抑制子）基因表达的影响。WSA 206（35 S：MPF 2-like-A）的过表达对开花有一定的促进作用，而WSB 206和TAB 201（35 S：MPF 2-like-B）的过表达对开花转变没有影响。同时，在35 S：WSA 206转基因植物中观察到SOC 1转录物丰度的升高和MAF 1转录物水平的降低。核苷酸多样性分析表明，在WSA 206蛋白的C-末端的一个非凡的8个氨基酸的延伸。在拟南芥中，没有这8个aa的截短的WSA 206版本（WSA 206 Delta C246）和由这8个aa延长的MPF 2样B版本（WSB 206 a双匕首C257和TAB 201 a双匕首C257）的异位表达揭示了8个aa标记在花转变中的模糊作用。它可能会影响蛋白质调节开花时间的能力，但对于早期开花来说既不充分也不是绝对必要的。然而，8个氨基酸的延伸影响了MPF 2样衍生物构建体中SOC 1和MAF 1的表达。我们的研究通过与SOC 1和MAF 1基因的相互作用，深入了解MPF 2样基因在相变中的作用，从而也指出了它们作为未来修饰作物开花的潜在候选者的意义。
A complex and intricate network of genes responding to various developmental and environmental signals control floral transition in plants. MADS-box genes are the key regulators and major contributors with regard to flowering time determination. Previously, MPF2-like genes belonging to the STMADS11 superclade were duplicated into MPF2-like-A and MPF2-like-B in Withania (WSA206 and WSB206) and Tubocapsicum (TAB 201). The present study was conducted to determine the effect of MPF2-like genes on flowering time by analyzing 35S:MPF2-like transgenic Arabidopsis plants as well as to probe their effects on the expression of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1, a floral promoter) and MADS AFFECTING FLOWERING 1 (MAF1, a floral repressor) genes. The overexpression of WSA206 (35S:MPF2-like-A) moderately promoted flowering, while that of WSB206 and TAB 201 (35S:MPF2-like-B) exhibited no effects on floral transition. Concomitantly, an elevation in SOC1 transcript abundance and a reduction for MAF1 transcript levels were observed in 35S:WSA206 transgenic plants. Nucleotide diversity analysis indicated an extraordinary 8 aa extension at the C-terminus of the WSA206 protein. Ectopic expression of a truncated WSA206-version without these 8 aa (WSA206 Delta C246) and of MPF2-like-B-versions elongated by these 8 aa (WSB206a double dagger C257 and TAB 201a double dagger C257) in Arabidopsis revealed an ambiguous role of the 8 aa signature in floral transition. It may influence a protein's ability to modulate flowering time but is neither sufficient nor strictly necessary for early flowering. Nevertheless, the 8 aa extension influences the expression of SOC1 and MAF1 in MPF2-like derivative constructs. Our studies provide insight into the role of MPF2-like genes in phase transition by interacting with SOC1 and MAF1 genes, thereby also pointing to their significance as potential candidates for modifying flowering in crop plants in the future.