The Drosophila centrosome-associated protein CP190 is essential for viability but not for cell division
The Drosophila centrosome-associated protein CP190 is essential for viability but not for cell division
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DOI：
10.1242/jcs.00979
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发表时间：
2004-03-01
影响因子：
4
通讯作者：
Whitfield, WGF
中科院分区：
文献类型：
--
作者：
Butcher, RDJ;Chodagam, S;Whitfield, WGF
果蝇的CP190和CP60蛋白相互作用，并在有丝分裂时在细胞核间期和中心体之间穿梭。这两种蛋白在体外均可直接与微管结合，并已被证明与唾液腺多烯染色体上的特定位点模式相关，但其功能尚不清楚。本研究表明，在组织培养细胞中，将CP190或CP60的水平降低50%或90%不会显著干扰中心体或微管组织、细胞分裂或细胞活力。然而，CP190是一种必需的蛋白质，因为CP190基因突变的纯合子果蝇在蛹发育后期死亡。在Cp190突变体的幼虫大脑中，有丝分裂不会从根本上受到干扰，Cp190的突变形式（CP190DeltaM）不能与微管结合或与中心体结合，可以挽救与Cp190基因突变相关的致命性。因此，CP190在果蝇中起着独立于中心体或微管的重要作用。
The Drosophila CP190 and CP60 proteins interact with each other and shuttle between the nucleus in interphase and the centrosome in mitosis. Both proteins can bind directly to microtubules in vitro, and have been shown to associate with a specific pattern of loci on salivary gland polytene chromosomes, but their functions are unknown. Here we show that reducing the level of CP190 or CP60 by >90% in tissue culture cells does not significantly interfere with centrosome or microtubule organisation, with cell division, or with cell viability. However, CP190 is an essential protein, as flies homozygous for mutations in the Cp190 gene die at late pupal stages of development. In larval brains of Cp190 mutants, mitosis is not radically perturbed, and a mutated form of CP190 (CP190DeltaM), that cannot bind to microtubules or associate with centrosomes, can rescue the lethality associated with mutations in the Cp190 gene. Thus, CP190 plays an essential role in flies that is independent of its association with centrosomes or microtubules.