Magnetic alginate microfibers as scaffolding elements for the fabrication of microvascular-like structures
Magnetic alginate microfibers as scaffolding elements for the fabrication of microvascular-like structures
复制标题
磁性藻酸盐微纤维作为支架元件用于制造微血管样结构
DOI：
10.1016/j.actbio.2017.11.038
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发表时间：
2018
影响因子：
9.7
通讯作者：
Fukuda Toshio
中科院分区：
文献类型：
--
作者：
Sun Tao;Shi Qing;Huang Qiang;Wang Huaping;Xiong Xiaolu;Hu Chengzhi;Fukuda Toshio
传统的细胞包封支架可能会引起不良的宿主反应和细胞分布的不均匀性。因此，最近已经使用用于微支架掺入的细胞自组装的制造技术来产生用于自下而上构建血管样结构的环形细胞模块。微支架在促进组织形成方面显示出优势。然而，由于缺乏环形细胞微支架，它仍然是一个挑战，工程微尺度环形细胞模块（micro-TCM）制造微血管样结构。在这里，磁性藻酸盐微纤维（MAM）被用作支架元件，其中缠绕策略使它们能够形成微环作为环形细胞微支架。研究了这些微环的NIH/3 T3成纤维细胞生长，作为表面化学和MAM尺寸的函数。然后在三维微环表面形成NIH/3 T3成纤维细胞和细胞外基质层，成功制备了微中药。采用简单的非接触式磁性组装技术将微中药沿着微柱堆叠，然后通过细胞融合将组装好的微中药快速连接成微血管样结构。包封在MAMs中的内皮细胞或药物可以包括在微血管样结构中，作为用于血管组织工程的体外细胞模型，或作为未来药物测试的小型化平台。重要性声明磁性藻酸盐微纤维作为支架元件用于引导微尺度环形细胞模块中的细胞生长（微型TCMs），并为微型TCMs提供磁性功能，用于在外部磁场中进行非接触式3D组装。通过使用液体/空气界面，在液体环境中进行微TCM的非接触式空间操作，有效的电动电磁针。构建微血管样结构的新生物制造范例。微管状结构允许细胞与细胞直接接触，解决了细胞封装支架的问题。
Traditional cell-encapsulating scaffolds may elicit adverse host responses and inhomogeneity in cellular distribution. Thus, fabrication techniques for cellular self-assembly with micro-scaffold incorporation have been used recently to generate toroidal cellular modules for the bottom-up construction of vascular-like structures. The micro-scaffolds show advantage in promoting tissue formation. However, owing to the lack of annular cell micro-scaffolds, it remains a challenge to engineer micro-scale toroidal cellular modules (micro-TCMs) to fabricate microvascular-like structures. Here, magnetic alginate microfibers (MAMs) are used as scaffolding elements, where a winding strategy enables them to be formed into micro-rings as annular cell micro-scaffolds. These micro-rings were investigated for NIH/3T3 fibroblast growth as a function of surface chemistry and MAM size. Afterwards, micro-TCMs were successfully fabricated with the formation of NIH/3T3 fibroblasts and extracellular matrix layers on the three-dimensional micro-ring surfaces. Simple non-contact magnetic assembly was used to stack the micro-TCMs along a micro-pillar, after which cell fusion rapidly connected the assembled micro-TCMs into a microvascular-like structure. Endothelial cells or drugs encapsulated in the MAMs could be included in the microvascular-like structures asin vitrocellular models for vascular tissue engineering, or as miniaturization platforms for pharmaceutical drug testing in the future.Statement of SignificanceMagnetic alginate microfibers functioned as scaffolding elements for guiding cell growth in micro-scale toroidal cellular modules (micro-TCMs) and provided a magnetic functionality to the micro-TCMs for non-contact 3D assembly in external magnetic fields.By using the liquid/air interface, the non-contact spatial manipulation of the micro-TCMs in the liquid environment was performed with a cost-effective motorized electromagnetic needle.A new biofabrication paradigm of construct of microvascular-like structure. The micro-tubal-shaped structures allowed direct cell-to-cell contact that solved problems of cell-encapsulating scaffolds.