Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs.
Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs.
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MALDI-TOF MS作为诊断从猪的临床标本中回收的链球菌物种的诊断工具的有用性。
DOI：
10.1371/journal.pone.0170784
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Fernández-Garayzábal JF
中科院分区：
文献类型：
--
作者：
Pérez-Sancho M;Vela AI;García-Seco T;González S;Domínguez L;Fernández-Garayzábal JF
应用MALDI-TOF MS技术对临床分离的猪链球菌进行鉴定。对于该建议，MALDI BDAL数据库（Bruker Daltoniks，德国）补充了S. porci，S. porcorum和S.与肺炎和败血症相关的plurextorum。虽然这三个物种显示出相似的MALDI图谱，但识别出几个峰可用于它们的区分：S。porci（4113、6133、7975和8228 m/z Da）、S. plurextorum（3979、4078、4665、6164、6491、6812、7959和9330 m/z Da）和S. porcorum（3385、3954、4190、6772、7908和8381 m/z Da）。在添加这些MSP后，进行评价以确定MALDI-TOF MS用于使用74个田间分离株鉴定来自病猪的链球菌的准确性。分离株鉴定为S. suis，S. porcinus，S. dysgalactiae、S. hyovagelia，S. porcorum，S. alactolyticus、解乳链霉菌S.猪链球菌和S. orisratti这是第一次从猪的临床标本中报告后三种。总体而言，MALDI-TOF MS鉴定结果（最佳提示）与基因分型结果之间具有良好的一致性（95.9%）。我们的研究结果表明，MALDI-TOF MS的良好性能（100%的灵敏度和特异性），以确定大多数的链球菌，可以经常从患病的猪分离。然而，在正确鉴定某些S分离株时观察到相互矛盾的结果。dysgalactiae和S.溶乳菌
The application of MALDI-TOF MS for identifying streptococcal isolates recovered from clinical specimens of diseased pigs was evaluated. For this proposal, the MALDI BDAL Database (Bruker Daltoniks, Germany) was supplemented with the main spectrum profiles (MSP) of the reference strains of S. porci, S. porcorum and S. plurextorum associated with pneumonia and septicemia. Although these three species showed similar MALDI profiles, several peaks were recognized that can be useful for their differentiation: S. porci (4113, 6133, 7975 and 8228 m/z Da), S. plurextorum (3979, 4078, 4665, 6164, 6491, 6812, 7959 and 9330 m/z Da) and S. porcorum (3385, 3954, 4190, 6772, 7908, and 8381 m/z Da). After adding these MSPs, an evaluation was conducted to determine the accuracy of MALDI-TOF MS for the identification of streptococci from diseased pigs using 74 field isolates. Isolates were identified as S. suis, S. porcinus, S. dysgalactiae, S. hyovaginalis, S. porcorum, S. alactolyticus, S. hyointestinalis and S. orisratti. This is the first time that the latter three species have been reported from clinical specimens of pigs. Overall, there was good concordance (95.9%) between the results obtained from MALDI-TOF MS identification (best hint) and those from genotyping. Our results demonstrate the good performance of MALDI-TOF MS (100% sensitivity and specificity) for identifying most of the species of streptococci that can frequently be isolated from diseased pigs. However, conflicting results were observed in the correct identification of some isolates of S. dysgalactiae and S. alactolyticus.