Performance analyses of a neutralizing agent combination strategy for the production of succinic acid by Actinobacillus succinogenes ATCC 55618
Performance analyses of a neutralizing agent combination strategy for the production of succinic acid by Actinobacillus succinogenes ATCC 55618
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产琥珀酸放线杆菌 ATCC 55618 生产琥珀酸的中和剂组合策略的性能分析
DOI：
10.1007/s00449-011-0644-6
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发表时间：
2012-05-01
影响因子：
3.8
通讯作者：
Tang, Ya-Jie
中科院分区：
文献类型：
--
作者：
Wang, Cheng-Cheng;Zhu, Li-Wen;Tang, Ya-Jie
开发了一种中和剂组合策略以提高产琥珀酸放线杆菌ATCC 55618的琥珀酸产量。首先，在培养pH为7.5时，获得了最高为48.2 g/L的琥珀酸产量。其次，对NaOH和KOH进行筛选以确定用于pH控制的最佳中和剂。然而，当仅使用一种中和剂控制pH时，由于金属离子浓度较高，琥珀酸产量没有增加，并且观察到严重的细胞絮凝现象。最后，开发了一种供应含OH⁻和碳酸盐的中和剂的组合策略。细胞絮凝现象消除，使用5 M的NaOH和40 g/L的MgCO₃时，获得了最高为59.2 g/L的琥珀酸产量；该产量比单独使用NaOH时高27.9%。本研究中获得的结果可能对琥珀酸的大规模工业化生产有用。
A neutralizing agent combination strategy was developed to enhance the succinic acid production by Actinobacillus succinogenes ATCC 55618. First, a maximal succinic acid production of 48.2 g/L was obtained at a culture pH of 7.5. Second, NaOH and KOH were screened to identify the optimal neutralizing agent for pH control. However, the production of succinic acid did not increase, and severe cell flocculation was observed due to a high concentration of metal ions when only one neutralizing agent was used to control pH. Finally, a neutralizing agent combination strategy was developed with a supply of neutralizing agents with OH− and carbonate. The cell flocculation was eliminated, and a maximum succinic acid production of 59.2 g/L was obtained with 5 M NaOH and 40 g/L of MgCO3; this production was 27.9% higher than that obtained with NaOH alone. The results obtained in this study may be useful for the large-scale industrial production of succinic acid.