Analysis of the late steps of exocytosis: biochemical and total internal reflection fluorescence microscopy (TIRFM) studies.
Analysis of the late steps of exocytosis: biochemical and total internal reflection fluorescence microscopy (TIRFM) studies.
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胞吐作用后期步骤的分析：生化和全内反射荧光显微镜 (TIRFM) 研究。
DOI：
10.1007/s10571-006-9049-5
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发表时间：
2006
影响因子：
4
通讯作者：
Holz,RonaldW
中科院分区：
文献类型：
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作者：
Holz,RonaldW
1. 20 世纪 70 年代初期，朱莉在美国国立卫生研究院 (NIH) 的实验室与朱莉一起度过的时光被铭记。这段经历让我对儿茶酚胺分泌的调节产生了终生的兴趣。以下是这项工作的总结。 2．综述了 ATP 依赖性胞吐作用启动与多磷酸肌醇之间的关系。此外，还总结了使用全内反射荧光显微镜（TIRFM）观察胞吐作用之前的分泌颗粒行为和个体胞吐事件的研究。3．定量光学分析表明嗜铬颗粒的运动受到高度限制但受到调节。颗粒在融合前的 100 毫秒内可以经历显着的运动，并且在这段时间内可能发生与质膜的相互作用，从而导致融合。小的运动可以允许邻近质膜的颗粒重复采样质膜的微区，从而增加导致融合的有效相互作用的可能性。
1. Time with Julie in his laboratory at the NIH in the early 1970s is remembered. The experience led to a life-long interest in the regulation of catecholamine secretion. Here are summarized aspects of this work.2. The relationship between ATP-dependent priming of exocytosis and the polyphosphoinositides is reviewed. In addition, studies are summarized in which total internal reflection fluorescent microscopy (TIRFM) was used to visualize secretory granule behavior before exocytosis and individual exocytotic events.3. Quantitative optical analysis indicates that chromaffin granule motion is highly restricted but regulated. Granules can undergo significant motion in the 100 ms prior to fusion and interactions with the plasma membrane leading to fusion can occur within this time. The small motions may permit granules adjacent to the plasma membrane to repetitively sample microdomains of the plasma membrane, thereby increasing the probability of fruitful interactions that lead to fusion.