A portable multi-wavelength fiber-based quantitative phase microscope for imaging dispersive samples
A portable multi-wavelength fiber-based quantitative phase microscope for imaging dispersive samples
复制标题
用于成像色散样品的便携式多波长光纤定量相显微镜
DOI：
10.1117/12.2579051
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
R. Zhou
中科院分区：
文献类型：
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作者：
Mengxuan Niu;R. Zhou
我们展示了一种新的便携式多波长基于光纤的定量相位显微镜（CQPM）。在双折射相位调制中，参考光束在单模光纤中传播，单模光纤的长度被调谐以确保与样品光束的最佳干涉对比度。为了进一步紧凑，不同的照明波长通过光纤组合器和耦合器复用。该方法可以同时获得两个波长的干涉图，并分别得到相应的相位图。我们已经使用CNOPM成像色散样品，如荧光颗粒和红细胞，并进一步分析折射率和细胞内血红蛋白浓度。
We demonstrate a new portable multi-wavelength fiber-based quantitative phase microscope (FQPM). In FQPM, the reference beam is made to propagate in a single-mode optical fiber whose length is tuned to ensure the best interference contrast with the sample beam. For further compactness, different illumination wavelengths are multiplexing through fiber combiners and couplers. FQPM simultaneously acquires the sample interferograms coming from two illumination wavelengths in a single shot, and their corresponding phase maps can be obtained separately. We have used FQPM for imaging dispersive samples, such as fluorescence particles and red blood cells and further analyze the refractive index and intracellular hemoglobin concentration.