Methyl jasmonate attenuated lipopolysaccharide-induced depressive-like behaviour in mice
Methyl jasmonate attenuated lipopolysaccharide-induced depressive-like behaviour in mice
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DOI：
10.1016/j.jpsychires.2017.06.007
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发表时间：
2017-11-01
影响因子：
4.8
通讯作者：
Umukoro, Solomon
中科院分区：
文献类型：
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作者：
Adebesin, Adaeze;Adeoluwa, Olusegun A.;Umukoro, Solomon
抑郁症是一种复发性神经精神疾病，影响着全球数百万人，并对患者的社会功能和生活质量产生负面影响。研究表明，腹腔注射脂多糖（LPS）通过诱导氧化应激和神经炎症诱导啮齿动物的抑郁样行为。茉莉酸甲酯（MJ）是一种从茉莉花植物中分离的化合物，在芳香疗法中因治疗抑郁、紧张和记忆缺陷而闻名。本研究旨在评价MJ对LPS诱导的小鼠抑郁样行为的影响。小鼠连续7天腹腔注射MJ（5-20 mg/kg）、丙咪嗪（10 mg/kg）或溶媒（10 mL/kg）。在第7天，在腹膜内注射LPS（830 μ g/kg）之前30分钟进行处理。LPS给药后24小时，进行悬尾、强迫游泳和蔗糖偏好试验。此后，使用ELISA测定血清皮质酮水平。测定脑组织匀浆中丙二醛（MDA）、谷胱甘肽（GSH）和肿瘤坏死因子-α（TNF-α）的水平。当与载体相比时，LPS显著增加尾部悬吊和强迫游泳测试中的不动时间（p < 0.05），这表明抑郁样综合征。然而，当与LPS处理组相比时，MJ（5-20 mg/kg）显著减少增加的不动时间。LPS给药还改变了小鼠体内MDA、GSH、皮质酮和TNF α的水平，MJ可显著逆转该变化。这些发现表明MJ对LPS诱导的抑郁样行为的减弱可能与抑制氧化应激和TNF α的释放有关。(C)2017爱思唯尔有限公司版权所有
Depression is a recurrent neuropsychiatric disorder that affects millions of individuals worldwide and impact negatively on the patients' social functions and quality of life. Studies have shown that i.p injection of lipopolysaccharide (LPS) induces depressive-like behavior in rodents via induction of oxidative stress and neuroinflammation. Methyl jasmonate (MJ), an isolated compound from jasmine plant has gained reputation in aromatherapy for treatment of depression, nervousness and memory deficits. This study was designed to evaluate the effects of MJ on LPS-induced depressive-like behavior in mice. Mice were given MJ (5-20 mg/kg), imipramine (10 mg/kg) or vehicle (10 mL/kg) intraperitoneally for 7 consecutive days. On day 7, treatment was carried out 30 min prior to i.p injection of LPS (830 mu g/kg). Twenty four hours after LPS administration, tail suspension, forced swim and sucrose preference tests were carried out. Thereafter, serum corticosterone levels were determined using ELISA. The levels of malondialdehyde (MDA), glutathione (GSH) and tumor necrosis factor-alpha (TNF-alpha) were determined in brain tissue homogenates. LPS significantly increased immobility time in the tail suspension and forced swim tests when compared with vehicle (p < 0.05), which indicates depressive-like syndromes. However, the increased immobility time was significantly reduced by MJ (5-20 mg/kg) when compared with LPS-treated group. LPS administration also altered the levels of MDA, GSH, corticosterone and TNF alpha in mice, which was significantly reversed by MJ. These findings suggest that attenuation of LPS-induced depressive-like behavior by MJ may be related to suppression of oxidative stress and release of TNF alpha. (C) 2017 Elsevier Ltd. All rights reserved.