Antimicrobial protein produced by vaginal Lactobacillus acidophilus that inhibits Gardnerella vaginalis.
Antimicrobial protein produced by vaginal Lactobacillus acidophilus that inhibits Gardnerella vaginalis.
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DOI：
10.1155/s1064744901000060
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发表时间：
2001-01-01
影响因子：
--
通讯作者：
Faro, S
中科院分区：
文献类型：
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作者：
Aroutcheva, A A;Simoes, J A;Faro, S
目的：从一株阴道嗜酸乳杆菌中分离细菌素。方法：嗜酸乳杆菌160在两种培养基上生长。第一次是MRS肉汤培养18小时；细胞被收获，清洗，并放入化学定义的培养液中。第二种培养液类似于阴道液中没有蛋白质。用硫酸铵从两种介质中沉淀细菌素。结果：MRS肉汤与吐温80结合，不适合细菌素的提取。细菌素是从化学定义的介质中分离出来的，没有污染成分，并通过电泳法鉴定为单一条带。细菌素的相对分子质量为3.8 kDa。从嗜酸乳杆菌160分离到的细菌素对9株加德纳氏菌均有抑制作用。结论：嗜酸乳杆菌160产生的细菌素是以部分纯的形式从化学规定的培养基中分离出来的。嗜酸乳杆菌160细菌素对9株阴道加德纳氏菌均有生长抑制作用。
OBJECTIVE: To isolate bacteriocin from a vaginal strain of Lactobacillus acidophilus.METHODS: L. acidophilus 160 was grown on two media. The first was MRS broth for 18 hours; the cells were harvested, washed, and placed into a chemically defined medium. The second medium resembled vaginal fluid minus protein. Bacteriocin was precipitated from both media using ammonium sulfate. The growth-inhibiting activity of bacteriocin was determined by a bioassay using nine different isolates of Gardnerella vaginalis.RESULTS: MRS broth is not a suitable medium for extracting bacteriocin, because it binds with Tween 80. Bacteriocin was isolated, without contaminating constituents, from chemically defined medium and identified as a single band by electrophoresis. Bacteriocin has a molecular weight of 3.8 kDa. All nine isolates of Gardnerella were inhibited by the bacteriocin isolated from L. acidophilus 160.CONCLUSIONS: Bacteriocin produced by L. acidophilus 160 was isolated from the chemically defined medium (starvation medium) in a partially pure form. L. acidophilus 160 bacteriocin inhibited growth of all nine isolates of Gardnerella vaginalis.