Grape seed proanthocyanidin extract inhibits interleukin-17-induced interleukin-6 production via MAPK pathway in human pulmonary epithelial cells
Grape seed proanthocyanidin extract inhibits interleukin-17-induced interleukin-6 production via MAPK pathway in human pulmonary epithelial cells
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DOI：
10.1007/s00210-011-0633-y
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发表时间：
2011-06-01
影响因子：
3.6
通讯作者：
Ha, Eunyoung
中科院分区：
文献类型：
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作者：
Kim, Hyeongseok;Kim, Jin Young;Ha, Eunyoung
本研究旨在探讨葡萄籽原花青素提取物（GSPE）对白细胞介素-17（IL-17）诱导的人肺上皮细胞A549产生白细胞介素-6（IL-6）的影响。用IL-17（10 ng/ml）或GSPE（50 μ g/ml）或两者处理细胞。评价GSPE对细胞活力和凋亡的影响。采用逆转录-聚合酶链反应和酶联免疫吸附试验分别检测IL-6的mRNA和蛋白水平。Western blot分析抑制因子κ B-α（I κ B-α）和ERK 1/2、p38和JNK丝裂原活化蛋白激酶（MAPK）的表达。GSPE对IL-17诱导的肺上皮细胞IL-6 mRNA和蛋白表达均有显著抑制作用（p < 0.01）。GSPE降低IL-17诱导的I κ B-α磷酸化。IL-17刺激ERK 1/2、p38和JNK MAPK活性，GSPE降低IL-17刺激的ERK 1/2、p38和JNK MAPK活性。GSPE还减弱TNF-α和IL-1 β诱导的IL-6产生（p < 0.05）。我们的研究结果表明，GSPE可能通过抑制MAPK和核因子-κ B介导的信号通路来抑制IL-17刺激的人肺上皮细胞产生IL-6。
This study was aimed to investigate the effect of grape seed proanthocyanidin extract (GSPE) on interleukin-17 (IL-17)-induced interleukin-6 (IL-6) production in A549 human pulmonary epithelial cells. Cells were treated with IL-17 (10 ng/ml) or GSPE (50 mu g/ml), or both. The effects of GSPE on cell viability and apoptosis were evaluated. The mRNA and protein levels of IL-6 were determined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Expressions of inhibitory factor kappa B-alpha (I kappa B-alpha) and ERK 1/2, p38, and JNK mitogen-activated protein kinase (MAPK) were examined by Western blot analysis. GSPE significantly inhibited both GSPE treatment only and IL-17-induced mRNA expressions and protein productions of IL-6 in pulmonary epithelial cells (p < 0.01). GSPE decreased the IL-17-induced phosphorylation of I kappa B-alpha. The IL-17 stimulated ERK 1/2, p38, and JNK MAPK activities and GSPE decreased IL-17-stimulated ERK 1/2, p38, and JNK MAPK activities. GSPE also attenuated TNF-alpha and IL-1 beta-induced IL-6 productions (p < 0.05). Our results show that GSPE may inhibit IL-17-stimulated IL-6 productions in human pulmonary epithelial cells by inhibiting MAPK and nuclear factor-kappa B-mediated signaling pathway.