Noninvasive Cathodoluminescence-Activated Nanoimaging of Dynamic Processes in Liquids
Noninvasive Cathodoluminescence-Activated Nanoimaging of Dynamic Processes in Liquids
复制标题
液体动态过程的非侵入性阴极发光激活纳米成像
DOI：
10.1021/acsnano.7b06081
复制
发表时间：
2017
期刊：
影响因子：
17.1
通讯作者：
Aloni, Shaul
中科院分区：
文献类型：
--
作者：
Bischak, Connor G.;Wai, Rebecca B.;Cherqui, Charles;Busche, Jacob A.;Quillin, Steven C.;Hetherington, Craig L.;Wang, Zhe;Aiello, Clarice D.;Schlom, Darrell G.;Aloni, Shaul
在这种情况下，电子显微镜提供了非常高的空间分辨率，但电子束辐照往往会破坏软材料，扰乱动态过程，要求样品非常坚固。在这里，我们取而代之的是使用共振能量转移阴极发光激活成像(Claire)，以亚衍射分辨率非侵入性地成像金属和聚合物纳米颗粒在液体环境中的动力学。在克莱尔，当受到低能量聚焦电子束的激发时，一个独立的闪烁体薄膜可以作为纳米级的光激发源。我们捕获了这些粒子沿闪烁体表面平移和解吸的纳米级动力学，并演示了50ms的帧捕获和距离闪烁体表面至少20 nm的成像范围。此外，在情景电子显微镜中，Claire提供了光谱选择性，而不是仅依赖于散射。我们还通过定量建模证明了金属纳米粒子的Claire信号受到多等离子体模干扰的影响。我们的研究结果表明，Claire是一种有前途的、无创的、具有高时空分辨率的软和流体材料的超分辨率成像方法。
In situelectron microscopy provides remarkably high spatial resolution, yet electron beam irradiation often damages soft materials and perturbs dynamic processes, requiring samples to be very robust. Here, we instead noninvasively image the dynamics of metal and polymer nanoparticles in a liquid environment with subdiffraction resolution using cathodoluminescence-activated imaging by resonant energy transfer (CLAIRE). In CLAIRE, a free-standing scintillator film serves as a nanoscale optical excitation source when excited by a low energy, focused electron beam. We capture the nanoscale dynamics of these particles translating along and desorbing from the scintillator surface and demonstrate 50 ms frame acquisition and a range of imaging of at least 20 nm from the scintillator surface. Furthermore, in contrast within situelectron microscopy, CLAIRE provides spectral selectivity instead of relying on scattering alone. We also demonstrate through quantitative modeling that the CLAIRE signal from metal nanoparticles is impacted by multiplasmonic mode interferences. Our findings demonstrate that CLAIRE is a promising, noninvasive approach for super-resolution imaging for soft and fluid materials with high spatial and temporal resolution.