Aberrant expression of type I fibroblast growth factor receptor in human pancreatic adenocarcinomas.
Aberrant expression of type I fibroblast growth factor receptor in human pancreatic adenocarcinomas.
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发表时间：
1993-10
期刊：
影响因子：
11.2
通讯作者：
M. Kobrin;Y. Yamanaka;H. Friess;Martha E. Lopez;M. Korc
中科院分区：
文献类型：
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作者：
M. Kobrin;Y. Yamanaka;H. Friess;Martha E. Lopez;M. Korc
酸性和碱性成纤维细胞生长因子是在胰腺癌中过表达的促有丝分裂多肽。为了确定成纤维细胞生长因子是否可以在体内对胰腺癌细胞产生直接影响，我们比较了人胰腺组织中高亲和力I型成纤维细胞生长因子受体（FGFR-1）的表达。在正常胰腺中，FGFR-1免疫染色主要见于腺泡细胞。在胰腺癌中，FGFR-1在导管样癌细胞中含量丰富，导管样癌细胞也显示出许多FGFR-1 mRNA原位杂交颗粒。通过聚合酶链反应和RNA酶保护的分析显示，FGFR-1的2-免疫球蛋白样和3-免疫球蛋白样形式在所有组织样品中表达，并且通过与正常组织相比，2-免疫球蛋白样形式在癌组织中过表达。这些发现表明FGFR-1的β 2-免疫球蛋白样形式可能有助于胰腺癌中异常的自分泌和旁分泌途径。
Acidic and basic fibroblast growth factors are mitogenic polypeptides that are overexpressed in pancreatic cancer. To determine whether fibroblast growth factors may exert direct effects on pancreatic cancer cells in vivo, we compared the expression of the high-affinity type I fibroblast growth factor receptor (FGFR-1) in human pancreatic tissues. In the normal pancreas, FGFR-1 immunostaining was seen mainly in acinar cells. In pancreatic cancers, FGFR-1 was abundant in ductal-like cancer cells which also exhibited many FGFR-1 mRNA in situ hybridization grains. Analysis by the polymerase chain reaction and RNase protection revealed that the 2-immunoglobulin-like and the 3-immunoglobulin-like forms of FGFR-1 were expressed in all tissue samples, and that the 2-immunoglobulin-like form was overexpressed in the cancer tissues by comparison with the normal tissues. These findings suggest that the 2-immunoglobulin-like form of FGFR-1 may contribute to aberrant autocrine and paracrine pathways in pancreatic cancer.