miR-125b Inhibits Connexin43 and Promotes Glioma Growth
miR-125b Inhibits Connexin43 and Promotes Glioma Growth
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DOI：
10.1007/s10571-013-9980-1
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发表时间：
2013-11-01
影响因子：
4
通讯作者：
Zhao, Gang
中科院分区：
文献类型：
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作者：
Jin, Zheng;Xu, Songbai;Zhao, Gang
microRNA与肿瘤的生长和发育密切相关。本研究探讨了miR-125 b在胶质瘤生长中的潜在作用。我们发现miR-125 b在体外能够促进胶质瘤细胞系的生长和克隆形成，并能保护胶质瘤细胞免于凋亡。miR-125 b转染的胶质瘤细胞在体内移植后也表现出生长增加。我们进一步证实了miR-125 b抑制了Connexin 43的表达，并且Connexin 43的过表达拮抗了miR-125 b在细胞生长和抗凋亡中的作用。我们的结论是，miR-125 b调节胶质瘤生长部分通过连接蛋白43蛋白。
MicroRNA is strongly associated with tumor growth and development. This study examined the potential roles of miR-125b in glioma growth. We found that miR-125b promotes glioma cell line growth and clone formation, and protects the glioma cells from apoptosis in vitro. The miR-125b-transfected glioma cells also demonstrated increased growth after in vivo transplantation. We further identified that miR-125b inhibits Connexin43 expression, and the overexpression of Connexin43 antagonizes the effects of miR-125b in cell growth and anti-apoptosis. We conclude that miR-125b regulates glioma growth partly through Connexin43 protein.