Molecular evolutionary relationships of enteroinvasive Escherichia coli and Shigella spp.
Molecular evolutionary relationships of enteroinvasive Escherichia coli and Shigella spp.
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DOI：
10.1128/iai.72.9.5080-5088.2004
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发表时间：
2004-09-01
影响因子：
3.1
通讯作者：
Reeves, PR
中科院分区：
文献类型：
--
作者：
Lan, R;Alles, MC;Reeves, PR
肠侵袭性大肠杆菌（EIEC）是大肠杆菌的一种特殊致病形式。引起痢疾的大肠杆菌在许多特性上与志贺氏菌四种中的细菌相似。志贺氏菌已被分离为一个属，但事实上包括几个克隆的E。杆菌通过对志贺菌属12个血清型的32株EIEC菌株的4个管家基因和2个质粒基因进行测序，确定了它们的进化关系。EIEC菌株分为四个簇，其中一个离群菌株，表明EIEC的独立衍生多次。四个簇中的三个含有一种以上的O抗原类型。在志贺氏菌3群中发现一株EIEC菌株（O 112 ac：H-菌株），但与志贺氏菌3群D2和B15菌株不同，具有相同的O抗原。通过使用ipgD和nixiA基因的序列，在志贺菌菌株中鉴定了两种形式的毒力质粒pINV，并且除了两种之外，我们的EIEC菌株都具有pINV A。EIEC菌株分为两个亚群，变异水平非常低，通常不与志贺氏菌pINV A菌株混合。基于管家基因的EIEC簇反映在质粒基因序列中，但有一些例外。通过使用ipgD序列发现两个菌株为pINV B形式，其中一个菌株具有与D1的趋异序列相似的nixiA序列。显然，EIEC和志贺氏菌属（Shigella spp.）形成E.杆菌
Enteroinvasive Escherichia coli (EIEC), a distinctive pathogenic form of E. coli causing dysentery, is similar in many properties to bacteria placed in the four species of Shigella. Shigella has been separated as a genus but in fact comprises several clones of E. coli. The evolutionary relationships of 32 EIEC strains of 12 serotypes have been determined by sequencing of four housekeeping genes and two plasmid genes which were used previously to determine the relationships of Shigella strains. The EIEC strains were grouped in four clusters with one outlier strain, indicating independent derivation of EIEC several times. Three of the four clusters contain more than one O antigen type. One EIEC strain (an O112ac:H- strain) was found in Shigella cluster 3 but is not identical to the Shigella cluster 3 D2 and B15 strains with the same O antigen. Two forms of the virulence plasmid pINV have been identified in Shigella strains by using the sequences of ipgD and nixiA genes, and all but two of our EIEC strains have pINV A. The EIEC strains were grouped in two subclusters with a very low level of variation, generally not intermingled with Shigella pINV A strains. The EIEC clusters based on housekeeping genes were reflected in the plasmid gene sequences, with some exceptions. Two strains were found in the pINV B form by using the ipgD sequence, with one strain having an nixiA sequence similar to the divergent sequence of D1. Clearly, EIEC and Shigella spp. form a pathovar of E. coli.