Synergy in thrombin-graphene sponge for improved hemostatic efficacy and facile utilization
Synergy in thrombin-graphene sponge for improved hemostatic efficacy and facile utilization
复制标题
凝血酶-石墨烯海绵的协同作用可提高止血功效并易于使用
DOI：
10.1016/j.colsurfb.2017.10.021
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发表时间：
2018-01-01
影响因子：
5.8
通讯作者：
Wang, Xing
中科院分区：
文献类型：
--
作者：
Li, Guofeng;Quan, Kecheng;Wang, Xing
复合材料因其潜在的协同效应而具有吸引力，这种协同效应可以带来高性能，但这种协同效应取决于微妙的设计。在这项研究中，一种止血复合材料，凝血酶/交联石墨烯海绵（TCGS），通过一个简单的梯度复合策略开发。CGS的多孔结构确保凝血酶稳定嵌入TCGS中，避免爆发释放，但保持其生物活性。在适当的凝血酶刺激和海绵的快速吸收的协同作用下，TCGS表现出出色的止血性能，在100秒内超快止血，优于CGS和等量的天然凝血酶。凝血酶剂量过低或过高会延长出血时间。研究表明，在界面处平衡血浆吸收和凝血酶刺激对提高止血效果至关重要。即使在环境中储存6个月后，TCGS也具有生物安全性和稳定性。这种潜在的超长保质期有利于其实际应用。因此，TCGS不仅为开发止血复合材料提供了新的策略，也为止血材料的设计提供了新的方法和认识。(C) 2017 Elsevier B.V.版权所有
Composites are attractive for its potential synergistic effects that can result in high-performance, but the synergy depends on subtle design. In this study, a hemostatic composite, a thrombin/cross-linked graphene sponge (TCGS), was developed through a facile gradient composite strategy. The porous structure of the CGS assures that the thrombin is stably embedded in the TCGS, avoiding a burst release but maintaining its bioactivity. In the synergy between proper thrombin stimulation and the fast absorption of the sponge, TCGS exhibits outstanding hemostatic performance, ultrafast bleeding cessation, within 100 s, which is superior to both CGS and equal amounts of native thrombin. Lower or excessive thrombin dosages prolong the bleeding time. The study revealed that the balance between plasma absorption and thrombin stimulation at the interface is critical for improving hemostatic efficacy. TCGS is also highlighted for its biosafety and stability, even after 6 months of storage in environment. This potentially ultra-long shelf life is conducive to its practical applications. Therefore, TCGS not only provides a new strategy for developing a hemostatic composite but also provides a new method and understanding for the design of hemostatic materials. (C) 2017 Elsevier B.V. All rights reserved.