Generation of nonidentical compartments in vesicular transport systems
Generation of nonidentical compartments in vesicular transport systems
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DOI：
10.1083/jcb.200409087
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发表时间：
2005-01-17
影响因子：
7.8
通讯作者：
Rapoport, TA
中科院分区：
文献类型：
--
作者：
Heinrich, R;Rapoport, TA
细胞器如何通过双向囊泡运输进行通信，同时保持不同的蛋白质组成？我们表明，通过数学建模，一个最小的系统，其中的基本变量是细胞溶质外套囊泡出芽和膜结合的可溶性N-乙基-马来酰亚胺敏感因子附着蛋白受体（SNARE）囊泡融合，是足以产生稳定的，不相同的车厢。建立和维持不同的隔室的要求是，每个涂层优先包装某些SNARE囊泡出芽期间。囊泡优先与含有最高浓度的同源SNARE的隔室融合，从而进一步增加这些SNARE。稳定的稳态是这种自催化SNARE在隔室中的积累和通过囊泡出芽在隔室之间的SNARE分布之间的平衡的结果。由此产生的非均匀的陷阱分布产生的涂层特定的囊泡通量，决定了车厢的大小。以这种方式建立的不相同的隔间，货物蛋白的定位和细胞运输可以简单地解释为它们对外套的亲和力。
How can organelles communicate by bidirectional vesicle transport and yet maintain different protein compositions? We show by mathematical modeling that a minimal system, in which the basic variables are cytosolic coats for vesicle budding and membrane-bound soluble N-ethyl-maleimide-sensitive factor attachment protein receptors (SNAREs) for vesicle fusion, is sufficient to generate stable, nonidentical compartments. A requirement for establishing and maintaining distinct compartments is that each coat preferentially packages certain SNAREs during vesicle budding. Vesicles fuse preferentially with the compartment that contains the highest concentration of cognate SNAREs, thus further increasing these SNAREs. The stable steady state is the result of a balance between this autocatalytic SNARE accumulation in a compartment and the distribution of SNAREs between compartments by vesicle budding. The resulting nonhomogeneous SNARE distribution generates coat-specific vesicle fluxes that determine the size of compartments. With nonidentical compartments established in this way, the localization and cellular transport of cargo proteins can be explained simply by their affinity for coats.