Dual effects of daidzein, a soy isoflavone, on catecholamine synthesis and secretion in cultured bovine adrenal medullary cells
Dual effects of daidzein, a soy isoflavone, on catecholamine synthesis and secretion in cultured bovine adrenal medullary cells
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DOI：
10.1210/en.2007-0073
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发表时间：
2007-11-01
期刊：
影响因子：
4.8
通讯作者：
Shinohara, Yuko
中科院分区：
文献类型：
--
作者：
Liu, Minhui;Yanagihara, Nobuyuki;Shinohara, Yuko
最近，我们证明了在培养的牛肾上腺髓质细胞质膜雌激素受体的发生和功能作用。本文报道大豆黄酮对细胞中儿茶酚胺合成和分泌的影响。用大豆苷元孵育细胞20分钟，以浓度依赖性方式（10-1000 nM）增加[C-14]酪氨酸而不是[C-14]二羟基苯丙氨酸合成C-14-儿茶酚胺。经典的雌激素受体抑制剂ICI 182，780不抑制大豆黄酮对C-14-儿茶酚胺合成的刺激作用。乙酰胆碱，一种生理促分泌素，刺激14 C-儿茶酚胺的合成，这是抑制大豆黄酮在1 μ M。大豆黄酮在高浓度（1-100 μ M）抑制由乙酰胆碱诱导的儿茶酚胺分泌。此外，大豆苷元（10-1000 nM）抑制[H-3] 17 β-雌二醇与从牛肾上腺髓质分离的质膜的特异性结合。目前的研究结果表明，大豆黄酮在低浓度下刺激通过质膜雌激素受体的儿茶酚胺合成，但在高浓度下抑制乙酰胆碱诱导的牛肾上腺髓质的儿茶酚胺合成和分泌。大豆黄酮的后一种作用可能是对心血管系统的有益作用。
We recently demonstrated the occurrence and functional roles of plasma membrane estrogen receptors in cultured bovine adrenal medullary cells. Here we report the effects of daidzein, a phytoestrogen of soybeans, on catecholamine synthesis and secretion in the cells. Incubation of cells with daidzein for 20 min increased the synthesis of C-14-catecholamines from [C-14] tyrosine but not [C-14] dihydroxyphenylalanine, in a concentration-dependent manner (10-1000 nM). The stimulatory effect of daidzein on C-14-catecholamine synthesis was not inhibited by ICI182,780, a classical estrogen receptor inhibitor. Acetylcholine, a physiological secretagogue, stimulated the synthesis of 14C-catecholamines, which was suppressed by daidzein at 1 mu M. Daidzein at high concentrations (1-100 mu M) suppressed catecholamine secretion induced by acetylcholine. Furthermore, daidzein (10-1000 nM) inhibited the specific binding of [H-3] 17 beta-estradiol to plasma membranes isolated from bovine adrenal medulla. The present findings suggest that daidzein at low concentrations stimulates catecholamine synthesis through plasma membrane estrogen receptors but at high concentrations inhibits catecholamine synthesis and secretion induced by acetylcholine in bovine adrenal medulla. The latter effect of daidzein may be a beneficial action on the cardiovascular system.