The mode of condensation of aspartic acid and dihydroxyacetone phosphate in quinolinate synthesis in Escherichia coli.
The mode of condensation of aspartic acid and dihydroxyacetone phosphate in quinolinate synthesis in Escherichia coli.
复制标题
大肠杆菌中天冬氨酸和磷酸二羟基丙酮缩合合成喹啉的模式。
DOI：
10.1016/0304-4165(77)90061-7
复制
发表时间：
1977
期刊：
影响因子：
--
通讯作者：
T. Scott
中科院分区：
文献类型：
--
作者：
F. Wicks;S. Sakakibara;R. Gholson;T. Scott
以[1- 14 C]葡萄糖为原料，经酶解制备了二羟基[3- 14 C]丙酮磷酸，并将其用作大肠杆菌喹啉酸合成酶的底物。碳-碳降解结果表明，96%的14 C位于碳-4上，表明在E.杆菌
Dihydroxy[3-14C]acetone phosphate was prepared enzymatically from [1-14C]glucose and used as a substrate in a partially purified quinolinate synthetase system prepared fromEscherichia colimutants. Carbon-by-carbon degradation of the resulting [14C]quinolinate showed that 96% of the14C was located in carbon-4, indicating that carbon-3 of dihydroxyacetone phosphate condenses with carbon-3 of aspartate in quinolinate synthesis inE. coli.