Optimization and scale-up of 2,3-butanediol production by Bacillus amyloliquefaciens B10-127
Optimization and scale-up of 2,3-butanediol production by Bacillus amyloliquefaciens B10-127
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DOI：
10.1007/s11274-011-0960-7
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发表时间：
2011-11
影响因子：
4.1
通讯作者：
Tao-wei Yang;Xian Zhang;Z. Rao;Shenghui Gu;Haifeng Xia;Zhenghong Xu
中科院分区：
文献类型：
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作者：
Tao-wei Yang;Xian Zhang;Z. Rao;Shenghui Gu;Haifeng Xia;Zhenghong Xu
研究了培养条件对2，3-丁二醇(2，3-BD)产量的影响及其放大的可能性。本实验使用了一株新分离的芽孢杆菌B10-127，该菌株属于GRAS微生物，具有显著的2，3-BD产生能力。根据Plackett-Burman实验设计结果，玉米浆、豆粕和柠檬酸铵是影响发酵的关键因素。用最陡上升路径法确定了3种因素的最佳浓度范围，并用响应面法进一步优化了3种因素的最佳浓度，分别为31.9g/L、22.0g/100g和5.58g/100g。优化后的培养基得率(62.7g/L)较优化前(45.7g/L)显著提高，2，3-BD产量提高约2.4倍(发酵时间由72小时缩短至42小时)。为了观察放大效果，在不同工作体积下进行了分批发酵。在L的装液量为20.0时，最终的2，3-BD浓度和产率分别为61.4g/g和0.38g/g，2，3-BD的产率为1.71g/L.h，这一结果表明在实验室规模发酵中获得了类似数量的2，3-BD，因此有可能放大到更大的发酵罐中，而不会出现大的问题。
The effects of culture conditions on 2,3-butanediol (2,3-BD) production and its possible scale-up have been studied. A newly isolatedBacillus amyloliquefaciensB10-127, belonged to GRAS microorganisms and showed a remarkable 2,3-BD producing potency, was used for this experiment. Corn steep liquor, soybean meal and ammonium citrate were found to be the key factors in the fermentation according to the results obtained from the Plackett–Burman experimental design. The optimal concentration range of the three factors was examined by the steepest ascent path, and their optimal concentration were further optimized via response surface methodological approach and determined to be 31.9, 22.0 and 5.58 g/l, respectively. The concentration of the obtained 2,3-BD increased significantly with optimized medium (62.7 g/l) when compared with unoptimized medium (45.7 g/l) and the 2,3-BD productivity was about 2.4-fold (The fermentation time was shorten from 72 to 42 h). To observe scale-up effects, batch fermentation was carried out at various working volumes. At a working volume of 20.0 l, the final 2,3-BD concentration and yield were 61.4 and 0.38 g/g at 36 h with a 2,3-BD productivity of 1.71 g/l h. This result shows similar amount of 2,3-BD obtained in lab-scale fermentation, and it is possible to scale up to larger fermentors without major problems.