Low photobleaching and high emission depletion efficiency: the potential of AIE luminogen as fluorescent probe for STED microscopy.
Low photobleaching and high emission depletion efficiency: the potential of AIE luminogen as fluorescent probe for STED microscopy.
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DOI：
10.1364/ol.40.002313
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发表时间：
2015-05
期刊：
影响因子：
3.6
通讯作者：
Jiaxin Yu;Xianhe Sun;F. Cai;Zhenfeng Zhu;A. Qin;J. Qian;Benzhong Tang;Sailing He
中科院分区：
文献类型：
--
作者：
Jiaxin Yu;Xianhe Sun;F. Cai;Zhenfeng Zhu;A. Qin;J. Qian;Benzhong Tang;Sailing He
我们提出了一个初步的研究，探讨潜在的聚集诱导发射（AIE）发光作为一种新的荧光探针STED显微镜。与STED显微镜中常用的有机荧光团Coumarin102相比，HPS是一种典型的AIE发光体，具有更强的抗光漂白能力。此外，掺杂HPS的纳米颗粒在有机溶液中具有比香豆素102更高的发射耗尽效率。AIE发光团的这两个优点可以促进STED显微镜中空间分辨率的提高以及长期成像。AIE发光团将是未来STED显微镜的一个有希望的候选者。
We present a preliminary study which explores the potential of aggregation-induced emission (AIE) luminogen as a new fluorescent probe for STED microscopy. Compared with Coumarin 102, which is a commonly used organic fluorophore in STED microscopy, HPS, a typical AIE luminogen, is more resistant to photobleaching. In addition, HPS-doped nanoparticles have higher emission depletion efficiency than Coumarin 102 in organic solution. These two advantages of AIE luminogen can facilitate the improvement of spatial resolution, as well as long-term imaging, in STED microscopy. AIE luminogen will be a promising candidate for STED microscopy in the future.