No Signature of Osteocytic Osteolysis in Cortical Bone from Lactating NMRI Mice
No Signature of Osteocytic Osteolysis in Cortical Bone from Lactating NMRI Mice
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DOI：
10.1007/s00223-019-00569-2
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发表时间：
2019-09-01
影响因子：
4.2
通讯作者：
Birkedal, Henrik
中科院分区：
文献类型：
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作者：
Wittig, Nina Kolln;Birkbak, Mie Elholm;Birkedal, Henrik
骨细胞与其他器官之间的信息交流作用已引起人们的广泛关注。长期以来，骨细胞是否和/或在多大程度上可以在称为骨细胞性骨溶解的过程中分解其周围的骨基质一直存在争议。研究表明，CD 1和C57 BL/6小鼠的骨细胞性骨质溶解是由多种骨骼挑战（包括哺乳）引起的，而固定诱导的骨细胞性骨质溶解仍然是一个有争议的问题。出于更好地理解这一过程的愿望，我们研究了哺乳期NMRI小鼠（一种广泛使用的远系小鼠品系）的骨细胞陷窝。令人惊讶的是，无论是通过同步加速器纳米断层扫描的3D研究，还是通过扫描电子显微镜的腔隙横截面积研究，还是通过光学显微镜，在胫骨或股骨皮质骨中均未检测到骨细胞性骨溶解的痕迹。这些结果使我们得出结论，骨细胞性骨质溶解不会发生在NMRI小鼠作为哺乳期的反应，反过来表明，骨细胞性骨质溶解可能不会在哺乳期动员钙发挥一般作用。
The roles of osteocytes in bone homeostasis have garnered increasing attention since it has been realized that osteocytes communicate with other organs. It has long been debated whether and/or to which degree osteocytes can break down the bone matrix surrounding them in a process called osteocytic osteolysis. Osteocytic osteolysis has been indicated to be induced by a number of skeletal challenges including lactation in CD1 and C57BL/6 mice, whereas immobilization-induced osteocytic osteolysis is still a matter of controversy. Motivated by the wish to understand this process better, we studied osteocyte lacunae in lactating NMRI mice, which is a widely used outbred mouse strain. Surprisingly, no trace of osteocytic osteolysis could be detected in tibial or femoral cortical bone either by 3D investigation by synchrotron nanotomography, by studies of lacunar cross-sectional areas using scanning electron microscopy, or by light microscopy. These results lead us to conclude that osteocytic osteolysis does not occur in NMRI mice as a response to lactation, in turn suggesting that osteocytic osteolysis may not play a generic role in mobilizing calcium during lactation.