In silico study on Arabidopsis BAG gene expression in response to environmental stresses.
In silico study on Arabidopsis BAG gene expression in response to environmental stresses.
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DOI：
10.1007/s00709-016-0961-3
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发表时间：
2017-01
期刊：
影响因子：
2.9
通讯作者：
Kang, Chang Ho
中科院分区：
文献类型：
--
作者：
Nawkar, Ganesh M.;Maibam, Punyakishore;Park, Joung Hun;Woo, Su Gyeong;Kim, Cha Young;Lee, Sang Yeol;Kang, Chang Ho
关键词：
BAG（Bcl-2 athanogene）家族蛋白在真核生物中广泛存在，并被认为在植物程序性细胞死亡（PCD）中起着重要作用。在过去的十年中，在先进的生物信息学工具的帮助下，已经在拟南芥基因组中确定了BAG基因的七个同源物;这些基因参与病原体攻击和非生物胁迫条件。在这项研究中，拟南芥BAG家族成员在环境胁迫下的基因表达进行了分析，使用植物阵列资源（BAR）表达浏览器工具，并在电脑数据部分确认的qRT-PCR分析所选的压力和水处理条件下的环境压力。特别地，通过使用GUS报告细胞系证实了AtBAG 6基因响应于热激的诱导。AtBAG 6基因的缺失导致植物基础耐热性受损，并在热胁迫下表现出增强的细胞死亡。为了阐明BAG基因的调控机制，我们分析了BAG 1-kbp启动子区域是否存在胁迫响应元件。我们的转录谱最终显示，拟南芥BAG基因的差异响应特定组织的上游调控元件的控制下，环境压力。本文的在线版本（doi：10.1007/s 00709 -016-0961-3）包含补充材料，可供授权用户使用。
BAG (Bcl-2 athanogene) family proteins are conserved in a wide range of eukaryotes, and they have been proposed to play a crucial role in plant programmed cell death (PCD). During the past decade, with the help of advanced bioinformatics tools, seven homologs of BAG genes have been identified in the Arabidopsis genome; these genes are involved in pathogen attack and abiotic stress conditions. In this study, gene expression of Arabidopsis BAG family members under environmental stresses was analyzed using the Botany Array Resource (BAR) expression browser tool and the in silico data were partially confirmed by qRT-PCR analysis for the selected stress- and hormone-treated conditions related to environmental stresses. Particularly, the induction of AtBAG6 gene in response to heat shock was confirmed by using GUS reporter lines. The loss of the AtBAG6 gene resulted into impairment in basal thermotolerance of plant and showed enhanced cell death in response to heat stress. To elucidate the regulatory mechanisms of BAG genes, we analyzed ∼1-kbp promoter regions for the presence of stress-responsive elements. Our transcription profiling finally revealed that the Arabidopsis BAG genes differentially respond to environmental stresses under the control of specifically organized upstream regulatory elements. The online version of this article (doi:10.1007/s00709-016-0961-3) contains supplementary material, which is available to authorized users.
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