Determination of release and uptake parameters from electrically evoked dopamine dynamics measured by real-time voltammetry.
Determination of release and uptake parameters from electrically evoked dopamine dynamics measured by real-time voltammetry.
复制标题
通过实时伏安法测量的电诱发多巴胺动力学确定释放和摄取参数。
DOI：
10.1016/s0165-0270(01)00459-9
复制
发表时间：
2001
影响因子：
3
通讯作者：
Garris,PA
中科院分区：
文献类型：
--
作者：
Wu,Q;Reith,ME;Wightman,RM;Kawagoe,KT;Garris,PA
通过神经递质多巴胺（DA）定量细胞外信号传导的潜在机制是一项困难的任务，特别是在完整大脑的复杂细胞外微环境中。在这项研究中，两种方法来评估释放和吸收DA动态监测实时伏安法。两者都是基于神经化学模型，其特征在于电诱发的DA水平作为这些对立机制之间的平衡。给出了这里所说的非线性回归和单曲线分析的理论基础。通过对模拟数据的拟合，验证了方法的可靠性。这两个分析还比较了一个实验数据集，描述的影响，抑制DA转运体的尾壳核（CP）和核神经节（NAC）。结果表明，非线性回归和单曲线分析是适合于定量的DA神经传递的释放和摄取机制。此外，这项技术研究最重要的实验发现是独立证实了完整纹状体中的高亲和力（> 0.2 μM）DA摄取。
Quantifying mechanisms underlying extracellular signaling by the neurotransmitter dopamine (DA) is a difficult task, particularly in the complex extracellular microenvironment of the intact brain. In this study, two methods for evaluating release and uptake from DA dynamics monitored by real-time voltammetry are described. Both are based on a neurochemical model characterizing electrically evoked levels of DA as a balance between these opposing mechanisms. The theoretical basis of what is called here nonlinear regression and single curve analyses is given. Fitting simulated data tests the reliability of the methods. The two analyses are also compared with an experimental data set describing the effects of pharmacologically inhibiting the DA transporter in the caudate-putamen (CP) and nucleus accumbens (NAc). The results indicate that nonlinear regression and single curve analyses are suitable for quantifying release and uptake mechanisms underlying DA neurotransmission. Additionally, the most important experimental finding of this technical study was the independent confirmation of high affinity (≈0.2 μM) DA uptake in the intact striatum.