Target-Responsive, DNA Nanostructure-Based E-DNA Sensor for microRNA Analysis
Target-Responsive, DNA Nanostructure-Based E-DNA Sensor for microRNA Analysis
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用于 microRNA 分析的目标响应、基于 DNA 纳米结构的 E-DNA 传感器
DOI：
10.1021/ac500251t
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发表时间：
2014-03-04
影响因子：
7.4
通讯作者：
Huang, Qing
中科院分区：
文献类型：
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作者：
Lin, Meihua;Wen, Yanli;Huang, Qing
由于microRNA的短尺寸和低丰度，开发快速，廉价和简单的生物传感器来检测它们具有挑战性。在这项工作中，我们已经展示了新一代（第三代）的E-DNA传感器的灵敏度和特异性检测microRNA。我们的第三代E-DNA传感器可以灵敏地检测低至1 fM的microRNA靶标（microRNA-141）。通过其对高度相似的microRNA类似物的区分能力，已经证明了其优异的特异性。在我们的设计中，DNA四面体的使用确保了茎环结构在良好控制的密度下具有改进的反应性。茎环结构的热力学稳定性的调节降低了背景信号，也增加了特异性。附着的酶带来电催化信号以放大检测。这些效应的组合提高了E-DNA传感器的灵敏度，使其适用于microRNA检测。最后，我们的第三代E-DNA传感器可推广到其他microRNA靶标（例如microRNA-21）的检测。
Because of the short size and low abundance of microRNAs, it is challenging to develop fast, inexpensive, and simple biosensors to detect them. In this work, we have demonstrated a new generation (the third generation) of E-DNA sensor for the sensitive and specific detection of microRNAs. Our third generation of E-DNA sensor can sensitively detect microRNA target (microRNA-141) as low as 1 fM. The excellent specificity has been demonstrated by its differential ability to the highly similar microRNA analogues. In our design, the use of DNA tetrahedron ensures the stem-loop structure in well controlled density with improved reactivity. The regulation of the thermodynamic stability of the stem-loop structure decreases the background signal and increases the specificity as well. The enzymes attached bring the electrocatalytic signal to amplify the detection. The combination of these effects improves the sensitivity of the E-DNA sensor and makes it suitable to the microRNA detection. Finally, our third generation of E-DNA sensor is generalizable to the detection of other micro RNA targets (for example, microRNA-21).