'Shocking' developments in chick embryology:: electroporation and in ovo gene expression
'Shocking' developments in chick embryology:: electroporation and in ovo gene expression
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DOI：
10.1038/70231
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发表时间：
1999-12-01
影响因子：
21.3
通讯作者：
Krumlauf, R
中科院分区：
文献类型：
--
作者：
Itasaki, N;Bel-Vialar, S;Krumlauf, R
在鸡蛋中通过电穿孔进行高效的基因转移，使得分析基因调控、功能和表达的新方法得以发展，创造了一个令人兴奋的机会，以建立在鸡胚胎系统的经典操纵优势的基础上。这种方法也适用于其他脊椎动物胚胎，是解决细胞和发育生物学问题的重要工具。本文介绍了体内电穿孔的技术特点、不同的应用领域和发展前景。
Efficient gene transfer by electroporation of chick embryos in ovo has allowed the development of new approaches to the analysis of gene regulation, function and expression, creating an exciting opportunity to build upon the classical manipulative advantages of the chick embryonic system. This method is applicable to other vertebrate embryos and is an important tool with which to address cell and developmental biology questions. Here we describe the technical aspects of in ovo electroporation, its different applications and future perspectives.