Characterization of rotavirus strains from hospitalized and outpatient children with acute diarrhoea in Sao Paulo, Brazil
Characterization of rotavirus strains from hospitalized and outpatient children with acute diarrhoea in Sao Paulo, Brazil
复制标题
DOI：
10.1002/jmv.20161
复制
发表时间：
2004-09-01
影响因子：
12.7
通讯作者：
Granato, CFH
中科院分区：
文献类型：
--
作者：
Carmona, RCC;Sampaio, MD;Granato, CFH
从1994年8月至1995年7月，从患有或未患有急性腹泻的儿童中收集了234个粪便样本并进行了测试。急性腹泻儿童组（A）又分为两个亚组：A亚组由严重腹泻、脱水和需要住院治疗的儿童组成，A（2）亚组由仅需要门诊治疗的儿童组成。B组为无急性腹泻的儿童（对照组）。A（1）、A（2）和B组中轮状病毒检出率分别为36.7%（18/49）、22.0%（15/68）和1.7%（2/117）。在35份阳性标本中，VP7基因型G1、G2、G3、G5和G2 + G5混合型分别占40.0%、11.4%、11.4%、22.9%和2.9%。VP4基因型P [8]、P [4]和P [6]的检出率分别为57.1%、31.4%和5.7%。轮状病毒VP6亚型I和11的检出率分别为22.4%和54.3%。轮状病毒RNA片段分别有20.0%和51.4%的病例出现短和长电泳图谱。疾病的严重程度与特定的G和P型、亚组或电泳分型无关。(C)2004 Wiley-Liss，Inc.
From August 1994 to July 1995, 234 faecal samples from children with or without acute diarrhoea were collected and tested. The group of children with acute diarrhoea (A) was subdivided into two subgroups: subgroup A, was made up of children with severe diarrhoea, dehydrated and who needed hospitalization and subgroup A(2) was composed of children who only needed outpatient care. Group B was composed of children without acute diarrhoea (controls). Rotavirus was detected in 36.7% (18/49), 22.0% (15/68) and 1.7% (2/117) patients in groups A(1), A(2) and B, respectively. Of the 35 positive samples in which rotaviruses were detected the VP7 genotypes G1, G2, G3, G5 and the mixture (G2 + G5) were found in 40.0, 11.4, 11.4, 22.9 and 2.9% of the samples, respectively. Also, the VP4 genotypes P[8], P[4] and P[6] were detected in 57.1, 31.4 and 5.7%, respectively. Rotavirus VP6 subgroups I and 11 were detected at a frequency of 22.4 and 54.3%, respectively. Rotavirus RNA segments had short and long electrophoresis profiles in 20.0 and 51.4% of the cases, respectively. The severity of the disease was not related to a specific G and P types, subgroup or electropherotype. (C) 2004 Wiley-Liss, Inc.