Characterization of micromachined silicon membranes for immunoisolation and bioseparation applications
Characterization of micromachined silicon membranes for immunoisolation and bioseparation applications
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DOI：
10.1016/s0376-7388(99)00062-9
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发表时间：
1999-07-01
影响因子：
9.5
通讯作者：
Ferrari, M
中科院分区：
文献类型：
--
作者：
Desai, TA;Hansford, D;Ferrari, M
本文介绍了微加工技术在生物医学领域中的应用。通过利用微电子工业（MEMS）中常用的制造技术，可以制造具有良好控制和均匀孔径的膜，从而允许优化用于细胞免疫隔离和病毒过滤中的生物医学应用的膜参数。使用体和表面微机械加工来创建扩散膜，通过在硅上沉积和随后蚀刻牺牲层，已经获得了低至18 nm的孔径。研究表明，膜对氧气、葡萄糖和胰岛素等小生物分子具有足够的渗透性，同时排除了免疫球蛋白G（IgG）等较大蛋白质的通过。微加工膜的半渗透性、它们的生物相容性、易于灭菌、沿着它们的热稳定性和化学稳定性可以为生物医学应用提供显著的优势。微加工技术也可以应用于其他感兴趣的材料，以开发高度受控的膜。(C)1999 Elsevier Science B.V.保留所有权利。
The application of microfabrication technology to create precise separation and isolation membranes for biomedical applications is described. By utilizing fabrication techniques commonly employed in the microelectronics industry (MEMS), membranes can be fabricated with well-controlled and uniform pore sizes, allowing the optimization of membrane parameters for biomedical applications in cell immunoisolation and viral filtration. Using bulk and surface micromachining to create diffusion membranes, pore sizes down to 18 nm have been attained through deposition and subsequent etching of sacrificial layers on silicon. Membranes were shown to be sufficiently permeable to small biomolecules such as oxygen, glucose, and insulin, while excluding the passage of larger proteins such as immunoglobulin G (IgG). The semipermeability of microfabricated membranes, their biocompatibility, ease in sterilization, along with their thermal and chemical stability, may provide a significant advantages for biomedical applications. Microfabrication technology may also be applied to other materials of interest for the development of highly controlled membranes. (C) 1999 Elsevier Science B.V. All rights reserved.