Immunohistochemical detection of platinated DNA in the cochlea of cisplatin-treated guinea pigs
Immunohistochemical detection of platinated DNA in the cochlea of cisplatin-treated guinea pigs
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DOI：
10.1016/j.heares.2004.12.007
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发表时间：
2005-05-01
期刊：
影响因子：
2.8
通讯作者：
Smoorenburg, GF
中科院分区：
文献类型：
--
作者：
van Ruijven, MWM;de Groot, JCMJ;Smoorenburg, GF
顺铂诱导的耳毒性与Corti器、血管纹和螺旋神经节的功能和形态学变化相关。然而，耳蜗部位的顺铂吸收和积累尚未确定的属性。因此，我们已经开发了一种免疫组织化学方法，间接检测顺铂在半薄冷冻切片的豚鼠耳蜗（基转）使用的抗血清含有顺铂-DNA加合物的抗体。顺铂给药后，在Corti器和侧壁的大多数细胞的细胞核中存在铂化DNA。核免疫染色在外毛细胞、边缘细胞和螺旋韧带纤维细胞中最为明显。这项研究是第一次证明顺铂的存在下，在耳蜗的组织切片。(c)2004 Elsevier B. V.保留所有权利。
Cisplatin-induced ototoxicity is correlated with functional and morphological changes in the organ of Corti, the stria vascularis and the spiral ganglion. However, the cochlear sites of cisplatin uptake and accumulation have not been property identified. Therefore, we have developed an immunohistochemical method to, indirectly, detect cisplatin in semithin cryosections of the guinea pig cochlea (basal turn) using an antiserum containing antibodies against cisplatin-DNA adducts. Platinated DNA was present in the nuclei of most cells in the organ of Corti and the lateral wall after cisplatin administration. Nuclear immunostaining was most pronounced in the outer hair cells, the marginal cells and the spiral ligament fibrocytes. This study is the first to demonstrate the presence of cisplatin in histological sections of the cochlea. (c) 2004 Elsevier B.V. All rights reserved.