Two Distinct Regulatory Systems Control Pulcherrimin Biosynthesis in Bacillus subtilis.
Two Distinct Regulatory Systems Control Pulcherrimin Biosynthesis in Bacillus subtilis.
复制标题
两个不同的调控系统控制枯草芽孢杆菌中的普切里明生物合成。
DOI：
10.1101/2024.01.03.574033
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Simmons,LyleA
中科院分区：
文献类型：
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作者：
Fernandez,NicolasL;Simmons,LyleA
转录调控是一个基本的过程，允许细菌以适当的时间和响应幅度响应外部刺激。在土壤细菌枯草芽孢杆菌中，转录调控是细胞生存所需的发育过程的核心。从指数期过渡到稳定期的细胞中的基因表达受到一组称为过渡态调节因子（TSRs）的转录因子的控制。TSR影响许多发育过程，包括生物膜形成和运动性，遗传能力和孢子形成之间的决定，但TSR影响细菌生理学的程度仍有待充分阐明。在这里，我们证明了两个TSRs，ScoC和AbrB，沿着与MarR家族转录因子PchR负调节生产的铁螯合剂pulcherrimin在枯草杆菌。三个转录因子之间的关系的遗传分析表明，都是必要的，以限制在指数期的生产，并影响率和生产的Pulcherrimin总量。类似地，发现Pulcherrimin生物合成基因vmC的表达受ScoC、AbrB和PchR的控制，并且与由每种背景产生的Pulcherrimin的量相关。最后，我们的体外数据表明，在控制pulcherrimin生产沿着与AbrB和PchR的SCOC的弱直接作用。两个不同的监管系统的分层监管强调了pulcherrimin在B. subtilisphysiology的重要作用。
Regulation of transcription is a fundamental process that allows bacteria to respond to external stimuli with appropriate timing and magnitude of response. In the soil bacteriumBacillus subtilis, transcriptional regulation is at the core of developmental processes needed for cell survival. Gene expression in cells transitioning from exponential phase to stationary phase is under the control of a group of transcription factors called transition state regulators (TSRs). TSRs influence numerous developmental processes including the decision between biofilm formation and motility, genetic competence, and sporulation, but the extent to which TSRs influence bacterial physiology remains to be fully elucidated. Here, we demonstrate two TSRs, ScoC and AbrB, along with the MarR-family transcription factor PchR negatively regulate production of the iron chelator pulcherrimin inB.subtilis. Genetic analysis of the relationship between the three transcription factors indicate that all are necessary to limit pulcherrimin production during exponential phase and influence the rate and total amount of pulcherrimin produced. Similarly, expression of the pulcherrimin biosynthesis geneyvmCwas found to be under control of ScoC, AbrB, and PchR and correlated with the amount of pulcherrimin produced by each background. Lastly, ourin vitrodata indicate a weak direct role for ScoC in controlling pulcherrimin production along with AbrB and PchR. The layered regulation by two distinct regulatory systems underscores the important role for pulcherrimin inB.subtilisphysiology.