Copy number control of Tn5 transposition.
Copy number control of Tn5 transposition.
复制标题
Tn5 转座的拷贝数控制。
DOI：
10.1093/genetics/107.1.9
复制
发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Reznikoff,WS
中科院分区：
文献类型：
--
作者：
Johnson,RC;Reznikoff,WS
研究了含有一个或多个转座元件拷贝的大肠杆菌中 Tn5 的转座。研究发现，无论细胞中存在多少 Tn5 拷贝数，细胞内转座的总体频率都保持恒定。测量差异标记 Tn5 转座频率的实验证实，单个 Tn5 的转座频率与细胞中存在的元件拷贝总数成比例降低。 IS50R 编码的功能蛋白 2 先前已被证明是转座抑制剂，足以介导这种抑制作用。细胞中蛋白质 2 的浓度似乎可以调节单个 Tn5 元件的转座，从而使细胞中 Tn5 的总体转座保持恒定。
Transposition of Tn5inEscherichia colistrains containing one or multiple copies of the transposable element was investigated. It was found that the overall frequency of transposition within a cell remained constant regardless of the number of copies of Tn5present in that cell. Experiments measuring the transposition frequency of differentially marked Tn5s confirmed that the frequency of transposition of an individual Tn5decreased proportionally with the total number of copies of the element present in a cell. The IS50R-encoded function, protein 2, which has previously been shown to be an inhibitor of transposition, is sufficient to mediate this inhibitory effect. The concentration of protein 2 in a cell appears to modulate the transposition of individual Tn5elements in such a way that the overall transposition of Tn5in a cell remains constant.