Lactobacillus helveticus and Bifidobacterium longum taken in combination reduce the apoptosis propensity in the limbic system after myocardial infarction in a rat model
Lactobacillus helveticus and Bifidobacterium longum taken in combination reduce the apoptosis propensity in the limbic system after myocardial infarction in a rat model
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DOI：
10.1017/s0007114509990766
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发表时间：
2009-11-28
影响因子：
3.6
通讯作者：
Rousseau, Guy
中科院分区：
文献类型：
--
作者：
Girard, Stephanie-Anne;Bah, Thierno Madjou;Rousseau, Guy
心肌梗死(MI)模拟边缘系统中促炎症物质的释放，诱导细胞凋亡，促炎症细胞因子被认为是细胞凋亡的根本原因，尽管其机制目前尚不完全解释和/或了解。此外，抑郁可能会引起胃肠道紊乱，从而维持促炎细胞因子水平的升高。一些特定的益生菌制剂可以减轻应激引起的胃肠道问题和促炎/抗炎细胞因子的比例。因此，我们假设，预防性给予益生菌可能会减少MI后边缘系统的细胞凋亡倾向。成年雄性SD大鼠饮用益生菌(乳杆菌和长双歧杆菌)或安慰剂，连续4周，然后结扎大鼠冠状动脉左前支40min，造成急性心肌梗死。再灌注期72小时后处死大鼠，两组大鼠的脑梗塞面积无差异。与安慰剂组相比，益生菌组杏仁核(外侧和内侧)以及齿状回的Bax/Bcl-2(促凋亡/抗凋亡)比率和caspase-3(促凋亡)活性降低。AKT活性(抗凋亡)在相同的三个区域增加。结论：益生菌L与黄连预防治疗可降低脑梗塞后不同脑区细胞凋亡的易感性。
Myocardial infarction (MI) simulates the release of pro-inflammatory substances that induce apoptosis in the limbic system Pro-inflammatory cytokines are considered as the root cause of apoptosis, although the mechanism is not fully explained and/or understood at this time. In addition, depression may induce gastrointestinal perturbations that maintain the elevated levels of pro-inflammatory cytokines. It has been shown that some specific probiotic formulations may reduce gastrointestinal problems induced by stress and the pro/anti-inflammatory cytokine ratio Therefore, we hypothesised that probiotics, when given prophylactically, may diminish the apoptosis propensity in the limbic system following a MI. Male adult Sprague-Dawley rats were given probiotics (Lactobacillus helvencits and Bifidobacterium longum in combination) or placebo in their drinking-water for four consecutive weeks A MI was then induced in the rats by occluding the left anterior coronary artery for 40min. Rats were killed following a 72h reperfusion period Infarct size was not different in the two groups. Bax/Bcl-2 (pro-apoptotic/anti-apoptotic) ratio and caspase-3 (pro-apoptotic) activity were reduced in the amygdala (lateral and medial), as well as in the dentate gyrus in the probiotics group when compared with the placebo. Akt activity (anti-apoptotic) was increased in these same three regions. No significant difference was observed in Ca1 and Ca3 for the different markers measured In conclusion, the probiotics L helvetcus and B longum, given in combination a,, preventive therapy, reduced the predisposition of apoptosis found in different cerebral regions following a MI.