Modifications of dopamine D1 receptor complex in rats self-administering cocaine
Modifications of dopamine D1 receptor complex in rats self-administering cocaine
复制标题
DOI：
10.1016/s0014-2999(98)00731-6
复制
发表时间：
1998-11-27
影响因子：
5
通讯作者：
Smith, JE
中科院分区：
文献类型：
--
作者：
De Montis, MG;Co, C;Smith, JE
考马斯亮蓝是自发和实验性自我给药，当重复给药时，它诱导对先前评估的最小活性剂量的稳定形式的致敏作用。在本研究中，长期植入颈静脉导管的大鼠三联体的治疗方法如下：一只动物接受自我注射可卡因的训练，而另外两只被动接受可卡因或生理盐水，只要自我给药大鼠完成反应要求。反应稳定30天后，处死动物，测定不同脑区多巴胺D-1受体密度和腺苷酸环化酶活性。接受可卡因的动物（自我管理和轭）表现出下调多巴胺D-1受体的数量和多巴胺刺激腺苷酸环化酶的活性在延髓核，相比盐水大鼠。在嗅结节，多巴胺刺激腺苷酸环化酶活性出现选择性和显着下调自我管理的动物。(C)1998 Elsevier Science B. V.保留所有权利。
Cocaine is spontaneously and experimentally self-administered and, when given repeatedly, it induces a stable form of sensitization to a previously assessed minimum active dose. In the present study, triads of rats chronically implanted with a jugular catheter were treated as follows: one animal was trained to self-inject cocaine, while the other two passively received either cocaine or saline whenever the self-administering rat completed the response requirement. After 30 days of stable responding, the animals were sacrificed and dopamine D-1 receptor density and adenylyl cyclase activity were measured in different brain areas. Animals receiving cocaine (both self-administering and yoked) showed a down-regulation of dopamine D-1 receptor number and of dopamine stimulated adenylyl cyclase activity in the nucleus accumbens, as compared to saline rats. In the olfactory tubercle, dopamine stimulated adenylyl cyclase activity appeared selectively and significantly down-regulated in self-administering animals. (C) 1998 Elsevier Science B.V. All rights reserved.