ROLE OF SUPERMAN IN MAINTAINING ARABIDOPSIS FLORAL WHORL BOUNDARIES
ROLE OF SUPERMAN IN MAINTAINING ARABIDOPSIS FLORAL WHORL BOUNDARIES
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DOI：
10.1038/378199a0
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发表时间：
1995-11-09
期刊：
影响因子：
64.8
通讯作者：
MEYEROWITZ, EM
中科院分区：
文献类型：
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作者：
SAKAI, H;MEDRANO, LJ;MEYEROWITZ, EM
拟南芥基因 SUPERMAN (SUP) 对于生殖花组织的适当空间发育是必需的(1-3) 隐性突变导致在正常第三轮雄蕊内部形成额外的雄蕊，但以第四轮心皮发育为代价(1-3)。突变体表型与 B 功能基因 AP3 和 PI 在改变的花区中的异位表达相关，比野生型更靠近花的中心 (3)，并且 ap3sup 和 pisup 双突变体表现出与 ap3 和 pi 单突变体相似的表型。这些发现导致 SUP 被解释为 B 功能器官识别基因的上游负调节因子，作用于第四轮 (2,3)，以在雄蕊和心皮轮之间建立边界。在这里，我们通过分子克隆和分析表明，它在第三轮中表达，并在发育中的花朵中发挥维持这一边界的作用。假定的 SUPERMAN 蛋白含有一个锌指和一个类似于基本亮氨酸拉链基序的区域，表明其具有转录调节功能。
THE Arabidopsis gene SUPERMAN (SUP) is necessary for the proper spatial development of reproductive floral tissues(1-3) Recessive mutations cause extra stamens to form interior to the normal third whorl stamens, at the expense of fourth whorl carpel development(1-3). The mutant phenotype is associated with the ectopic expression of the B function genes, AP3 and PI, in the altered floral region, closer to the centre of the flower than in the wild type(3), and ap3 sup and pi sup double mutants exhibit a phenotype similar to ap3 and pi single mutants. These findings led to SUP being interpreted as an upstream negative regulator of the B function organ-identity genes, acting in the fourth whorl(2,3), to establish a boundary between stamen and carpel whorls. Here we show, using molecular cloning and analysis, that it is expressed in the third whorl and acts to maintain this boundary in developing flowers. The putative SUPERMAN protein contains one zinc-finger and a region resembling a basic leucine zipper motif, suggesting a function in transcriptional regulation.