[A metabonomic study on the treatment of diabetic nephropathy with traditional Chinese medicine tang-shen-fang].
[A metabonomic study on the treatment of diabetic nephropathy with traditional Chinese medicine tang-shen-fang].
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DOI：
10.3724/sp.j.1123.2011.00320
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发表时间：
2011-04
期刊：
影响因子：
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通讯作者：
Huan Yu;Li Li-Li;Qiong-lin Liang;Yi-ming Wang;Ping Li;G. Luo
中科院分区：
文献类型：
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作者：
Huan Yu;Li Li-Li;Qiong-lin Liang;Yi-ming Wang;Ping Li;G. Luo
建立了一种基于超高效液相色谱-飞行时间质谱（UP-LC/TOF MS）的血浆代谢指纹分析方法。采用主成分分析和正交投影潜在结构判别分析对所得数据进行分析。观察糖肾方治疗糖尿病肾病的临床疗效。与安慰剂对照组相比，TSF治疗3个月和6个月后发现显著变化。血浆中的19种代谢物被确定为潜在的生物标志物，包括脂质、脂肪酸和氨基酸。目前的代谢组学研究有助于把握TSF治疗过程中整体代谢网络的变化，验证其临床疗效，并有助于了解其作用机制。
An ultra performance liquid chromatography-time of flight mass spectrometry (UP-LC/TOF MS)-based method for plasma metabolic fingerprinting analysis was established. Acquired data were analyzed by principal component analysis and orthogonal projection to latent structure-discriminant analysis. The effect of tang-shen-fang (TSF) on the treatment of diabetic nephropathy patients was evaluated. Significant changes were found after 3 and 6 months' treatment of TSF compared with placebo controls. Nineteen metabolites in plasma were identified as potential biomarkers, including lipids, fatty acids and amino acids. The present metabonomic study is helpful to grasp the changes of global metabolic networks during the treatment of TSF and to testify its clinical efficacy and understand its action mechanism.