Control of assembly of Dictyostelium myosin and actin filaments.
Control of assembly of Dictyostelium myosin and actin filaments.
复制标题
盘基网柄菌肌球蛋白和肌动蛋白丝组装的控制。
DOI：
10.1101/sqb.1982.046.01.051
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Stryer,L
中科院分区：
文献类型：
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作者：
Spudich,JA;Kuczmarski,ER;Pardee,JD;Simpson,PA;Yamamoto,K;Stryer,L
1966年，Hatano和Oosawa证明肌动蛋白是非细胞黏菌多头绒泡菌的一种成分。在1966年至1975年期间，肌动蛋白和肌球蛋白被证明存在于基本上所有的真核细胞中（综述参见Pollard和Weihing 1974），并且获得了大量关于这些蛋白质在生长和发育的不同阶段的细胞定位的信息（综述参见Goldman et al. 1976）。虽然尚未通过遗传分析证明这些蛋白质是细胞运动所必需的，但它们在细胞中以丝状组装体的形式出现在正确的位置和正确的时间，以使特定形式的运动发生。这些丝状组装体的瞬时性质需要严格控制其组装和组织。大量的细胞成分参与这些控制是预期的，许多论文在这卷总结了最近的结果，从各种organis.A主要重点是肌动蛋白和肌球蛋白的生物化学和结构的研究从细胞黏菌Dictyosteelium discoideum分离的几个有趣的控制蛋白。这种真核细胞可以很容易地克隆，在化学成分确定的培养基中大量生长，并进行遗传操作以检查运动缺陷的突变体。此外，在生物体发育周期的一个阶段，细胞表现出对cAMP的趋化性。详细了解这种外部信号是如何耦合到细胞内收缩装置，使细胞在一个特定的方向移动是我们实验室正在做的工作的目标之一。我们在这里介绍我们最近的一些结果有关控制肌球蛋白和肌动蛋白丝的形成。
In 1966, Hatano and Oosawa demonstrated that actin is a component of the acellular slime mold Physarum polycephalum. In the years from 1966 to 1975, actin and myosin were shown to exist in essentially all eukaryotic cells (for review, see Pollard and Weihing 1974) and a wealth of information was obtained about the cellular localization of these proteins at different stages of growth and development (for review, see Goldman et al. 1976). Although it has not been proven by genetic analysis that these proteins are essential for cell motility, their appearance in the cell as filamentous assemblies occurs at the right place and at the right time for a particular form of movement to occur. The transient nature of these filamentous assemblies requires strict controls of their assembly and organization. A large number of cellular components involved in these controls are expected, and numerous papers in this volume summarize recent results on several interesting control proteins isolated from a variety of organisms.A primary emphasis of our laboratory has been biochemical and structural studies of actin and myosin from the cellular slime mold Dictyostelium discoideum. This eukaryotic cell can be easily cloned, grown in large amounts in a chemically defined medium, and manipulated genetically to examine mutants defective in motility. Furthermore, at one stage of the developmental cycle of the organism, the cells exhibit chemotaxis toward cAMP. A detailed understanding of how this external signal is coupled to the intracellular contractile apparatus to cause the cell to move in a particular direction is one goal of the work being done in our laboratory. We present here some of our recent results concerning control of myosin-and actin-filament formation.